A green and orange treefrog sat on a leaf.

Investigating vision: a wide-eyed tiger-striped leaf frog, Callimedusa tomopterna, in the forests of French Guiana © Dr Christian Cox

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The secrets of frog vision are finally being revealed

Discover how frogs see the world with Museum scientists.

Through a series of expeditions up mountains and into rainforests, experts are figuring out exactly how frogs' eyesight works, using a wide range of technologies in the process.

What do we know about frog vision?

Frogs are amphibians - animals that live between water and land. Their eyes have to deal with a lot compared with most of the animal kingdom.

Yet despite this incredible adaptability, surprisingly little research has delved into amphibian eyesight, and how they perceive the world around them.

Dr Jeff Streicher, Senior Curator of Amphibians and Reptiles at the Museum, says, 'Unlike other vertebrate groups such as mammals, birds and reptiles, we actually know very little about amphibian vision.'

Almost everything we know is based upon a few studies looking at a limited number of frogs. This creates a problem because frogs are incredibly diverse in what they get up to, from those that spend all their lives underwater, to some that pass their entire lives in the canopy, to others that live solely underground.

'There are almost 7,000 species of living frogs described so far, and they're not all the same,' explains Jeff.

'In other groups where we see large numbers of species that have evolved over tens to hundreds of millions of years, we know that visual systems have changed a lot, often in relation to what they're doing.' 

A small green frog calling with its throat expanded.

The forests of French Guiana support a wide range of frogs, including Hyalinobatrachium cappelli © Dr Christian Cox

Investigating frog eyesight in the wild

Scientists from the Museum, along with colleagues from the Smithsonian and the University of Texas at Arlington, have set out on a series of expeditions to the four corners of the world to investigate how frog eyes have changed as the animals have adapted to different lifestyles.

By looking at the amphibian family tree, the researchers were able to identify the most evolutionarily and ecologically distinctive groups. They then overlaid where these frogs live on a map of the world. This has taken the team to some of the world's most biodiverse places, stopping off in Australia, Gabon, and the Pacific Northwest coast of the United States of America.

The latest expedition took the researchers to the remote rainforests of French Guiana in South America. With near-pristine forests and a tropical ecosystem, it is an ideal place to find a wide range of frogs. 

How do frogs see in the dark?

Watch the video below to find out more.

How do frogs see in the dark?


Frogs in a rainforest laboratory

During their trips, the team have carried with them an impressive piece of equipment called a microspectrophotometer, built and run by Cornell University vision expert Ellis Loew.

This instrument is used to measure which wavelengths of light - ranging from ultraviolet to infrared - are absorbed by visual pigments in the rod and cone cells in the retinas of frogs. These two types of cells are known as photoreceptors, with the rods being responsible for vision in low light and the cones when light levels are higher. 

What do frogs see?

Researchers can determine how many visual pigments each frog has, and the range of wavelengths each frog species might then be able to perceive. In turn, this can help build up a picture of what the amphibians can see.

Carrying out this experiment in the field is easier said than done. It requires the complex kit to be kept at a stable room temperature and in complete darkness - not easy to achieve at a remote research station in the humid jungle. It also needs fresh retinal cells.

A low and log hut set against a background of rainforest.

The lodgings located in the middle of the rainforest © Dr Simon Maddock

To do this, the scientists must first humanely euthanise the amphibian. Then, carefully, the eye is removed and dissected so as not to damage the tissue, and the retina is mounted on a microscope slide.

Crucially, and as an added hurdle, this all has to be carried out in the dark so as not to activate the visual pigments in advance.

'You are putting the retinal tissue on the slide, and then that is taken and put into a microscope which is equipped with a very narrow beam that runs through all different wavelengths of light,' explains Jeff.

This beam is then fired at individual rod and cone cells in the retinal tissue, allowing the researchers to measure exactly which wavelengths are being absorbed, and to determine which types of pigments are present in each type of photoreceptor.  

Collecting frogs to save them

Fresh retinal tissue is used for other aspects of the project, such as studying which genes are expressed in the eyes of each frog.

Two researchers stood in a stream at night

Searching for amphibians can be easier at night © Dr Simon Maddock

This is done by analysing the different RNA found in the eye tissue, an indication of which proteins are being made and thus which genes are expressed in the eye. But RNA is not very stable.  

'RNA degrades very quickly after the animal stops making it,' says Jeff. Even with fresh tissue, the researchers need to use a special buffer to prevent the molecules from falling apart as soon as the animal dies. This means that tissues from Museum specimens are not feasible for looking at RNA.

'We're still a long way off from doing the same with Museum collections as what we can with the fresh tissues,' explains Jeff.

'So that's why we've got this hybrid approach where we're using some fresh collections, and then using both the Smithsonian and the Natural History Museum collections for the other bits, such as understanding how eye size has evolved among frogs.

'Fresh tissue collections are also of use for understanding the genetic diversity and other aspects of the natural history of frogs, especially poorly known tropical species. This is something that we actively do to give us a better chance of understanding these animals, and ultimately to protect them.'

Bright yellow and brown treefrog sat on a branch

The scientists hope to finally work out just how frogs, such as this clown treefrog, see the world. © Dr Christian Cox

Changing how we see amphibians

The Museum team, which also includes Dr David Gower, Head of Vertebrates Division, and Dr Kate Thomas, Postdoctoral Researcher, are looking at many aspects involved in how frogs see - from the ways in which they perceive light, to the genes expressed in the tissue and even the characteristics of the lens and shape of the eye.

'I think that the series of papers that come out of the project will recalibrate how we think about frog and amphibian vision,' says Jeff. 'But it's a case that we know so little that any extra information will do that.'

The researchers have one final stop off later this year, in Brazil, before turning their full attention to examining what the data can tell them about how amphibians see the world around them.