Last but not least, there was a time when a group of mite enthusiasts
(Goff, Tenorio, Brennan, and A. van Bronsjwick (sp.?)) got
together in the lab at Bishop Museum, decided to sample for Demodex.
What we did was fold a 3x5 index card into half and with the pointed edge
of the fold, we scraped
around the nose where it's oily, and above the eyebrows and then dip it in
Hoyer's on a slide, put cover slip, and voila!, Demodex were extracted.
You may have to scrape hard to get the tiny animals.

I have not done it recently, but I bet it would still work.
Sabina
____________________________________
Sabina F. Swift
Department of Plant and Environmental Protection Sciences
University of Hawaii at Manoa
3050 Maile Way, Gilmore 310
Honolulu, Hawaii 96822-2271
Phone: (808) 956-2445
Fax: (808) 956-2428
E-mail: sabina@hawaii.edu

On Fri, 28 Sep 2001, Demastes wrote:

> Gracious list members:
>
> Thank you to everyone who responded to my Demodex lab query.  I received
> many very useful ideas.  If anyone else is interested in the results, I
> have a word document with all of the responses. Just e-mail me and I
> will send it.
>
> -Jim
>
> --
> --------------------------
> James W. Demastes
> Department of Biology
> 2438 McCollum Science Hall
> University of Northern Iowa
> Cedar Falls, IA  50614
> Phone: (319) 273-2022
> Fax: (319) 273-7125
> --------------------------
CC: "acarology@nhm.ac.uk" <acarology@nhm.ac.uk>

I had my class take an insect pin and rub the head of the pin along their
hairline, then put the head in a drop of alcohol on a slide and you could
see the Demodex that were forced out of the follicles.  About half the
class found mites this way.

Richard
 

On Fri, 28 Sep 2001, Carlos H.W. Flechtmann wrote:

>
>
> ---------- Forwarded message ----------
> Date: Fri, 28 Sep 2001 08:23:54 -0300 (BRST)
> From: Carlos H.W. Flechtmann <chwflech@carpa.ciagri.usp.br>
> To: Demastes <jim.demastes@uni.edu>
> Subject: Re: Demodex demo?
>
> Dear Dr. Demastes
>
>  If yoy have access to pig skin (from a slaughterhouse):
>
>  - fix it in formol 10% for ca. 1-2 days
>  - pass to ethanol 70-%
>
>  - with a razor blade cut thin cross sections
>
>  - look for the sebaceous glands - rather large yellowish pouches
>
>  - scrape out their content
>
>  - put in a drop of Hoyer's medium, "dissolve"
>
>  - coverslip
>
>  - microscope - one usually sees hundreds of "microscopic
>   crocodiles" as they were once called
>
> From humans:
>
>  - examine the roots of eye lashes removed from the eye lids
>
>   (take 2-4 eyelashes from each lid, mount in Hoyer's)
>
>  - but, be careful - I do not know if the "plucking" of an
>   eyelash does not require a MD.
>
>      Carlos Flechtmann
>      University of Sao Paulo
>      Brasil
>
> On Thu, 27 Sep 2001, Demastes wrote:
>
> > Please excuse this non-research oriented question.  In an effort to try
> > and recruit freshmen into acarology (cospeciation in fur mites and
> > rodents) I am trying to incorporate a short activity (in a symbiosis
> > lab) that will let them search themselves for Demodex.  I have heard
> > about scraping a slide across the eyebrows but does anyone else have any
> > worked out protocols for this kind of gee whiz lab (ways to collect,
> > best lighting and magnification etc.).  I realize that only half (or
> > less) will have them, but even if we find a few it would be effective.
> > Thank you in advance.
> >
> > --
> > --------------------------
> > James W. Demastes
> > Department of Biology
> > 2438 McCollum Science Hall
> > University of Northern Iowa
> > Cedar Falls, IA  50614
> > Phone: (319) 273-2022
> > Fax: (319) 273-7125
> > --------------------------
> >
> >
> >
>
>
>
>
 
 
 
 

Richard Fagerlund, B.C.E.
Environmental Services             Home:  247 Mountain Shadows
University of New Mexico                  P.O. Box 1173
Albuquerque, NM 87131                     Corrales, NM 87048
E-mail: fagerlun@unm.edu                  (505) 922-9705 (home)
(505) 277-9904 (office)            (505) 440-6384 (cellular)
                      www.askthebugman.com
 
 
 

CC: <jim.demastes@uni.edu>, net Acarology <acarology@nhm.ac.uk>

---------- Forwarded message ----------
Date: Fri, 28 Sep 2001 08:23:54 -0300 (BRST)
From: Carlos H.W. Flechtmann <chwflech@carpa.ciagri.usp.br>
To: Demastes <jim.demastes@uni.edu>
Subject: Re: Demodex demo?

Dear Dr. Demastes

 If yoy have access to pig skin (from a slaughterhouse):

 - fix it in formol 10% for ca. 1-2 days
 - pass to ethanol 70-%

 - with a razor blade cut thin cross sections

 - look for the sebaceous glands - rather large yellowish pouches

 - scrape out their content

 - put in a drop of Hoyer's medium, "dissolve"

 - coverslip

 - microscope - one usually sees hundreds of "microscopic
  crocodiles" as they were once called

From humans:

 - examine the roots of eye lashes removed from the eye lids

  (take 2-4 eyelashes from each lid, mount in Hoyer's)

 - but, be careful - I do not know if the "plucking" of an
  eyelash does not require a MD.

     Carlos Flechtmann
     University of Sao Paulo
     Brasil

On Thu, 27 Sep 2001, Demastes wrote:

> Please excuse this non-research oriented question.  In an effort to try
> and recruit freshmen into acarology (cospeciation in fur mites and
> rodents) I am trying to incorporate a short activity (in a symbiosis
> lab) that will let them search themselves for Demodex.  I have heard
> about scraping a slide across the eyebrows but does anyone else have any
> worked out protocols for this kind of gee whiz lab (ways to collect,
> best lighting and magnification etc.).  I realize that only half (or
> less) will have them, but even if we find a few it would be effective.
> Thank you in advance.
>
> --
> --------------------------
> James W. Demastes
> Department of Biology
> 2438 McCollum Science Hall
> University of Northern Iowa
> Cedar Falls, IA  50614
> Phone: (319) 273-2022
> Fax: (319) 273-7125
> --------------------------

Gracious list members:

Thank you to everyone who responded to my Demodex lab query.  I received
many very useful ideas.  If anyone else is interested in the results, I
have a word document with all of the responses. Just e-mail me and I
will send it.

-Jim

--
--------------------------
James W. Demastes
Department of Biology
2438 McCollum Science Hall
University of Northern Iowa
Cedar Falls, IA  50614
Phone: (319) 273-2022
Fax: (319) 273-7125
--------------------------

Dear all,

Does anyone work on mite genetics?
I'm interested to find if anyone can help me with practical techniques for the measurement of genetic distance between mite populations.
As part of my PhD at Nottingham University, I am conducting experiments on the effectiveness of wildlife corridors using a moss / mite system. I am interested in the genetic differences between mites in unconnected moss patches and mites in moss patches connected by moss corridors.

Thanks for your attention,

Martin Hoyle.
 

From:  "D. CrossleyJr" <soilmite@earthlink.net>
To: <acarology@nhm.ac.uk>
Date:  9/25/01 4:50am
Subject:  Dermanyssus

Who's working with Dermanyssus these days?  I have a species berlesed from bluebird nests I'd like to have i.d.'d.

Thanks,

Dac

D. A. Crossley, Jr.
P. O. Box 903
Athens  GA  30603
706-543-0639

The Future:  The past returning through another gate.
   --  Arnold Glascow

Can anybody give me an e-mail address for Wayne Moss?

Thanks,

Dac

D. A. Crossley, Jr.
P. O. Box 903
Athens  GA  30603
706-543-0639

The Future:  The past returning through another gate.
   --  Arnold Glascow

I have found a homonym created by Mihelcic in the Damaeidae.  Does anyone
know if Mihelcic is still alive and active and how to contact him.
Otherwise, is there a specialist in this family who would be interested in
creating a replacement name for this genus

Joel Hallan
Austin, Texas

_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp
 

At 1:07 AM +0200 9/3/01, Jean-Bernard HUCHET wrote:
>Dear all,
>
>I would be very grateful if somebody could inform me about the taxonomic
>placement of the mites belonging to Glyciphagidae (order ?).
>
>Thank you very much in advance for the help,
>
The family Glycyphagidae (correct spelling) belongs to the superfamily
Glycyphagoidea, infraorder Astigmata, suborder Sarcoptiformes, order
Acariformes.

Dear all,

I would be very grateful if somebody could inform me about the taxonomic
placement of the mites belonging to Glyciphagidae (order ?).

Thank you very much in advance for the help,

Best whishes,

Jean-Bernard

In reply to Zhi-Qiang's comments, I was not suggesting that the archives be
deleted. The difference is that when people send a message they know it will
be archived and I assume it is harder to extract the addresses. Putting up a
simple list of addresses was asking for trouble, particularly with
"/listmember" in the page address. Subscribers to the list can always find
out who is on it by sending the "who acarology" command to
majordomo@nhm.ac.uk. Some junk mail is inevitable, but it can become
excessive for a "high-profile" address. I know this from past experience.
Eventually these get onto the lists of e-mail addresses that are sold
commercially. Even if spam mail is usually obvious (except if the subject
line is left blank), it takes a moment every day to sift through them all.
If there are viruses attached, it can slow things down more.

Having said that, I am sure we are all grateful Zhi-Qiang for a very useful
resource.

Best wishes,

Mark Judson

Dear Colleagues

  Would you please kindly send a copy of a paper to me as follows?

Subhan, F., Emery, M. & Shah, M.J. (1995) Hormonal profle of the extract of red mite Dinothrombium tinctorium. Hamdard Medicus, 38(2), 87-95.

   Thank you in advance for your kind considerations.

Best regards

Sincerely yours
Alireza Saboori, Ph.D.
Department of Plant Protection
College of Agriculture
Tehran University
Karaj-Iran

Dear Gina

 Try:

 Nutting, W.B., 1965 - Host-parasite relations: Demodicidae.
  Acarologia 7 (2):301-317.
 Nutting, W.B., 1976 - Hair follicle mites (Demodex spp) of medical
  and veterinary concern.
  Cornell Veterinarian 66(2):214-231.

 both papers present extense literature.

 English, F.P. & W.B. Nutting, 1981 - Feeding characteristics in
  demodectic mites of the eyelid.
  Australian Journal of Ophthalmology 9:311-313.

     Carlos Flechtmann
     CNPq-Brasil  researcher
     University of Sao Paulo

On Thu, 30 Aug 2001, Gina Marie wrote:

> Aloha all!
> I was wondering if anyone has information on Demodectic Mites:
>  Especially -Allergic reactions to their infestation on humans
> How to safely get rid of them - out of the hair follicles etc-
>
> Any advice or information would be greatly appreciated-
> Thanks!
> Gina Marie
> 808 732-4888
>
>
 
 
 

CC: <acarology@nhm.ac.uk>