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Dear acarologists,
Following some recent questions about mounting media, I am able to share
with you some information I compiled on this subject. Several years
ago I
did an informal survey of colleagues on their experiences with various
media. The results can be found in the archives of the acarology list,
at
this address:
http://www.nhm.ac.uk/hosted_sites/acarology/archive/summary.html
I hope this information is of some interest.
You may also be interested in this paper : M. S. Upton, 1993. Aqueous
gum-chloral slide mounting media: an historical review. Bull. Ent.
Res. 83:
267-274.
Bruce Halliday
***********************************************************
Dr. R. B. Halliday
CSIRO Entomology
GPO Box 1700
Canberra ACT 2601
Australia
Telephone (02) 6246 4085
Mobile 0438 543509
International Telephone (61) (2) 6246 4085
Fax (02) 6246 4000
International Fax (61) (2) 6246 4000
E-mail bruceh@ento.csiro.au
http://www.ento.csiro.au/research/natres/natres.html
***********************************************************
----------------------------------------------------------------------
This mail is sent to all participants of "Acarology List",
"Informations Apterygotologiques", and "Collembola ML" (name of
receiver is not coincide with your name because I am sending you
through Bcc.).
With this mail I would like to ask investigators who prepare
microscopic permanent slide preparation to answer a questionnaire
about method on making and preserving preparations. Please ignore
this mail, therefore, if you are not concerned microscopic
preparations. Further, please forgive me in case you receive
the same
questionnaire once again or more, since I have sent it to participants
of another mailing lists and members of some societies as well.
----------------------------------------------------------------------
Dear colleague
I beg your generosity to send you such a long mail without
introduction. I am working at a museum in Japan and studying
taxonomy
of pauropods.
As you know, pauropods are very tiny and I must make them into
microscopic preparations* when examine. Unfortunately, however,
slide
preparations mounted with Hoyer's medium -most popular mounting medium
among Japanese soil zoologists- are to be invaded by air within a
couple of years with high frequency, and not a few portion lose
taxonomic value in several years. Moreover, unlike "hard" creatures
such as oribatid mites, pauropods are so "soft" that I cannot remount
them even when they become dried up.
-----
* My present method of mounting and preserving pauropods
(1) Use a 32x24mm cover glass as a slide glass, for I have to examine
pauropods from both dorsal and ventral sides with
oil immersion
lens.
(2) Place a material, which was stored in 70-80% ethanol, onto the
"slide glass" mentioned above (excess ethanol was
removed with
filtre paper), drip a drop of Hoyer's medium.
(3) Put a 15mm circle cover glass over the material gently.
(4) Let preparation as it is for 2 weeks for drying; no special
apparatus such as a hot plate nor an incubator is
used.
(5) Seal (ring) with nail enamel (OVERCOAT, SHISEIDO).
(6) Mount preparation on an aluminium flame (same to one which was
used by excellent Danish proturan taxonomist, late
Prof. Dr.
S.L.TUXEN), store in a slide box (wooden 100 slides)
and put the
box on a flapless-shelf.
Consequently it is a serious problem for me to find much better
alternative method as soon as possible. At first I decide to
send a
questionnaire about method on making and preserving preparations to
investigators who seem to prepare small creatures, mainly arthropods,
into microscopic permanent slide preparation. I will plan how
I may
perform experiments to search for better methods by taking its results
into consideration.
I am most grateful if you kindly help me by replying this
questionnaire, preferably by the end of April, 2001.
I also provided questionnaire sheet in a Microsoft Word file at the
website: <http://homepage.mac.com/norihagino/prep_Q_E.html>.
Please
visit there and download a file if it is convenient for you -it will
make you a little easier in answering.
At last, Please be sure that some parts of your answer except for the
personal information will be presented in the form of oral report and/
or publication (sorry, probably in Japanese!).
I am sorry to bother you much but I am really look forward to hear
from you.
With my kindest regards.
Yours sincerely
Yasunori HAGINO
----------
Yasunori HAGINO
Natural History Museum & Institute, Chiba
955-2 Aoba-cho, Chiba-shi 260-8682, JAPAN
TEL: +81-43-265-3274 FAX: +81-43-266-2481
mailto:hagino@chiba-muse.or.jp
========== Begining of the QUESTIONNAIRE ==========
QUESTIONNAIRE ABOUT MAKING AND PRESERVING MICROSCOPIC PERMANENT PREPARATION
Hereafter I ask you about your method in making and preserving
microscopic permanent slide preparation. If you use several mounting
media, glass,sealing media, etc., please provide answers for one
method that you use most frequently.
(1) "#" indicates that it is an item of choices. Show your selection
in clear way:
eg.1. put some symbol such as "x" before the item(s) you select;
eg.2. delete all items other than your selection.
(2) "< >": fill in by your words.
Please be sure that some parts of your answer except for the personal
information will be presented in the form of oral report and/or
publication (sorry, probably in Japanese!).
I would be grateful if I receive your answer until the end of April,
2001.
======================================================================
1. Taxon (taxa) you make into preparation: < >
2. Style of preparation:
#Individual as a whole #Dissected
parts #Serial section
#Other < >
3. Treatment(s) before preparation:
#Fixation with ethanol <concentration %>
#Fixation with other <chemical used; concentration
%>
#Penetration <chemical used>
#Dying <chemical used>
#Dehydration <method>
#Other < > #No treatment
4. Mounting medium you are using
a. Name of medium
(for each medium with an asterisk, an
example of formula is
given as * in [ADDENDA] at the end of
this questionnaire):
#Hoyer's medium*
#Gum-chloral* #Heinze's medium*
#SWAN's medium*
#Canada Balsam #EUKITT (O.Kindler)
#EUPARAL (Chroma-Gesellshaft)
#Aquatex (Merck)
#Entellan (Merck)
#Entellan neu (Merck) #Other < >
b. How do you get it?:
#Prepared by yourself
#Prepared by other #Purchase
#Other < >
c. Reason for using that medium: < >
d. Any other comments on mounting medium...: <
>
5. Slide glass you are using
a. Type:
#Green slide
#White slide #Hollow slide
#Other extra treatment (frost,
coating, etc.) < >
b. Manufacturer: < >
c. Thickness: < > (unit: #mm
#inch #Other < >)
d. Reason for using that slide glass: < >
e. Any other comments on slide glass...: < >
6. Cover glass you are using
a. Shape: #Circle #Square
#Other < >
b. Size: < > (unit: #mm #inch
#Other < >)
c. Manufacturer: < >
d. Reason for using that cover glass: < >
e. Any other comments on cover glass...: < >
7. Drying preparation
a. Drying method:
#Natural drying <period>
#Use incubator <temperature, period>
#Use hot plate <temperature,
period> #Other < >
b. Reason for using that method: < >
c. Any other comments on drying preparation...: <
>
8. Sealing (ringing)
a. Sealing (ringing) medium you are using:
#Nail enamel<manufacturer,
product name, colour, etc.>
#Gryptal (no longer available?)
#Murrayite (The Biology Shop)
#HSR medium (Midori-juji)
#Other < >
#Do not seal (ring)
b. Reason for using that medium: < >
c. Sealing (ringing) once, twice, or more...:
#Once #Twice
<duration between first and second time >
#Other < >
d. Any other comments on sealing (ringing) medium...:
< >
9. Storing preparation
a. Container:
#Slide box #Other
< >
[b-d: for chooser of "Slide box" in a]
b. Material:
#Wooden #Plastics
or similar material #Other < >
c. Structure:
#Cover is hinged to body; folded
when store
#Cover is free from body; put
it onto body when store
#Other < >
d. Volume (number of slides par a box):
#100 #50
#25 #12 #Other < >
e. Reason for using that container: < >
f. Orientation of slides:
#Put slides flat, cover glass
upwards
#Put slides flat, cover glass
downwards
#Put slides vertically
g. Reason for using that orientation: < >
h. Storage place:
#Flapped shelf
#Flapless shelf #Desktop #Other <
>
i. Storage room:
#Special room such as a specimen
room
#Ordinary room such as a laboratory,
office, etc.
#Other < >
j. Moisture control in storage room:
#Moistening
#Do nothing #Desiccating
k. Any other comments on Storing...: < >
10. Ageing of preparation
a. Ageing appears in slides:
#No change #Colour
of mounting medium become darken
#Invasion of air
#Other < >
b. (for chooser of "No change" in a)
How long does it last in good condition,
as far as you know?
(eg. 50 years): < >
[c-e: for chooser of "Colour of mounting medium..." in
a]
c. Frequency to become darken (eg. 5 slides out of 10):
< >
d. Average period to begin to become darken (eg. 1 year):
< >
e. Average period to lose the value as a preparation (eg.
3
years): < >
[f-h: for chooser of Invasion of air" in a]
f. Frequency to be invaded by air (eg. 5 slides out of
10): < >
g. Average period to begin to be invaded by air (eg. 1
year): < >
h. Average period to lose the value as a preparation (eg.
3
years): < >
i. (for chooser of "Other" in a)
describe "symptom" in the same way as
c, d: < >
j. Any other comments on Ageing...: < >
11. Counteraction for ageing
(for answerer of either of c, d, e in 10.)
a. Is it possible to remount material when it damaged?:
#yes #No
[b,c: for chooser of "Yes" in a]
b. Method to soften mounting medium (eg. keep in a moistened
container such as a desiccator): <
>
c. Success rate of remounting:
#almost 100%
#over 50% #less than 50%
[d,e: for chooser of "No" in a]
d. Reason why it is impossible (eg. material is so weak
that it
break into separate parts): <
>
e. Action to prevent damage of preparation:
#Examine all slides periodically
<eg. once a year> and remount
if it begin to darken
#Examine all slides periodically
<eg. once a year> and seal
(ring) again if it begin
to dry
#Do nothing (give up when damaged)
#Other < >
f. Any other comments on Counteraction for ageing...:
< >
12. Any other comments on mounting and preserving method... (any
matter which is not included above items, how you
reached to your
present method, failure you experienced, comments
for the method
now I am adopting ["My present method of mounting
and preserving
pauropods" are given as ** in [ADDENDA] at the end
of this
questionnaire], etc.): < >
13. Give references you think valuable in considering mounting and
preserving method ("Literature concerning method
in making
microscopic preparation of small arthropods so far
I have known"
are given as *** in [ADDENDA]): < >
Thank you very much for taking the time to complete the questionnaire.
At last I would like to know your personal information; I may contact
you again by postal mail, FAX, or TEL as well as e-mail:
Name: < >
E-mail address: < >
Postal address: < >
FAX or TEL: <FAX: TEL:>
Thank you very much again for your kindest co-operation.
----------[ADDENDA]----------
* Examples of formulas for some mounting media
----------------------------
Hoyer's medium
----------------------------
distilled water ........ 25g
gum arabic ............. 15g
chloralhydrate ........ 100g
glycerin ............... 10g
----------------------------
----------------------------
Gum-chloral
----------------------------
gum arabic ............. 8g
chloralhydrate ......... 30g
distilled water ........ 10ml
acetic acid ............. 1ml
glycerin ................ 2ml
----------------------------
----------------------------
Heinze's medium
----------------------------
polyvinyl alcohol ...... 10g
lactic acid ............ 35cc
phenol(15%) ............ 25cc
glycerin ............... 10cc
chloralhydrate ......... 20g
distilled water .... 60@80cc
----------------------------
----------------------------
SWAN's medium
----------------------------
distilled water ........ 20g
gum arabic ............. 15g
chloralhydrate ......... 60g
glucose ................. 3g
acetic acid ............. 5g
----------------------------
** My present method of mounting and preserving pauropods
(1) Use a 32x24mm cover glass as a slide glass, for I have to examine
pauropods from both dorsal and ventral sides with
oil immersion
lens.
(2) Place a material, which was stored in 70-80% ethanol, onto the
"slide glass" mentioned above (excess ethanol was
removed with
filtre paper), drip a drop of Hoyer's medium.
(3) Put a 15mm circle cover glass over the material gently.
(4) Let preparation as it is for 2 weeks for drying; no special
apparatus such as a hot plate nor an incubator is
used.
(5) Seal (ring) with nail enamel (OVERCOAT, SHISEIDO).
(6) Mount preparation on an aluminium flame (same to one which was
used by excellent Danish proturan taxonomist, late
Prof. Dr.
S.L.TUXEN), store in a slide box (wooden 100 slides)
and put the
box on a flapless-shelf.
*** Literature concerning method in making microscopic preparation of
small arthropods so far I have known (ones written
in Japanese
omitted)
Dindal, L. D. (ed.), 1990. Soil Biology Guide. Wiley.
xiii +
1349 pp.
Evans, G. O., J. G. Sheals and D. Macfarlane, 1961. The terrestrial
Acari of the British Isles. vol. I. Brit.
Mus. (Nat. Hist.),
London, vii + 219 pp. [not obtained yet]
Gray, P., 1954. The microtomist's formulary and guide.
Blakiston.
xiii + 794 pp.
Grony, M. and L. Grum (ed.), 1993. Methods in Soil
Zoology.
Elsevier. xii+459 pp.
Krantz, G. W., 1978. A manual of acarology (2nd. ed.).
Oregon State
Univ. BookStores, Inc., Corvallis, viii + 509 pp.
[not obtained yet]
Martin, J. E. H. 1977. The Insects and Arachnids of Canada.
Part 1.
Collecting, Preparing, and Preserving Insects, Mites
and Spiders.
Publication 1643, Agriculture Canada. 182
pp.
Saito, Y. and Mh, Osakabe, 1992. A new fixation method for preparing
mite specimens for optical and SEM microscopic observations.
Appl. Entomol. Zool., 27: 427-436.
Saito, Y., Mh, Osakabe, Y. Sakagami and Y. Yasui, 1993. A method
for
preparing permanent specimens of mites with Canada
balsam. Appl.
Entomol. Zool., 28: 593-597.
Singer, G., 1967. A comparison between different mounting techniques
commonly employed in acarology. Acarologia,
9: 475-484.
Theron, J. G., 1958. Comparative studies on the morphology of
male
scale insects (Hemiptera: Coccoidea). Ann.
Univ. Stellenbosch,
34: 1-71 + 42 figs. [not obtained yet]
========== The end of the QUESTIONNAIRE ==========
****Please reply to: unlimited.power@connectfree.co.uk
From: unlimited.power@connectfree.co.uk
To: <zhangz@landcare.cri.nz>
Date: 3/29/01 9:32
Subject: Dermatophagoides cultures
Dear Dr Zhang
I came across your web page - and email address- while attempting to
find house dust mite culture methods. I am heading a research group working
on the involvement of human common cold viruses (mainly rhinoviruses) on
asthma. While these viruses are implicated in over 80% of asthma attacks,
no one knows the mechanisms of any possible synergy between these agents
and the house dust mites (mainly dermatophagoides spp) on the airway epithelium.
Dermatophagoides allergens have protease activities that may synergize
with rhinoviruses though the transcription factor NF-kB.
We are therefore planning to co-culture lung epithelial cells with
virus and dermatophagoides allergens with determined protease activities.
However, we are not familiar with the techniques of mite isolation and/or
culture, on which your expertise could be invaluable. We will be obliged
if you could guide us to either publications or dermatophagoides experts
that could help us in that respect.
Thanks in advance
Frankly
Nikolaos G. Papadopoulos, MD PhD
Allergy Unit
2nd Dpt of Pediatrics
University of Athens
On Wed, 28 Mar 2001, Cheol-Min Kim wrote:
> Dear Acarophiles,
>
> Long time ago, I excerpted the following quote from the source that
I
> do not recall right now.
>
>
> "Acarology is, in fact, in a state of systematic turmoil similar
to
> that experienced by entomologists a century ago." --- G. W. Krantz
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
It's in the Manual of Acarology (both editions) under Classification
(page
55 in the first edition and page 99 in the second).
Jerry Krantz
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
>
> I should be greatly appreciated if anyone in the net could give me
the
> citation. TIA.
>
> All the best, Cheol-Min Kim
>
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>
> Nature created species; Man created genera.
>
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>
> Cheol-Min Kim
>
> Acarology Laboratory (OSAL)
>
> Museum of Biological Diversity
>
> Ohio State University
>
> 1315 Kinnear Rd.
>
> Columbus, OH, 43212-1192, U.S.A.
>
>
> Tel: +1-(614) 292-7180
>
> Fax: +1-(614) 292-7774
>
> E-mail: Kim.296@osu.edu
CC: <acarology@nhm.ac.uk>
Dear Acarophiles,
Long time ago, I excerpted the following quote from the source that
I
do not recall right now.
"Acarology is, in fact, in a state of systematic turmoil similar to
that experienced by entomologists a century ago." --- G. W. Krantz
I should be greatly appreciated if anyone in the net could give me the
citation. TIA.
All the best, Cheol-Min Kim
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Nature created species; Man created genera.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Cheol-Min Kim
Acarology Laboratory (OSAL)
Museum of Biological Diversity
Ohio State University
1315 Kinnear Rd.
Columbus, OH, 43212-1192, U.S.A.
Tel: +1-(614) 292-7180
Fax: +1-(614) 292-7774
E-mail: Kim.296@osu.edu
Hello Tim,
You can find them in Thyrophagus, but they like Amblyseius cucumeris
and
Amblyseius degenerans eggs.
Kris
-----Original Message-----
From: Zhi-Qiang Zhang [mailto:ZhangZ@landcare.cri.nz]
Sent: donderdag 22 maart 2001 22:25
To: acarology@nhm.ac.uk
Subject: fwd:Rearing Neoseiulus barkeri on Tyrophagus putrescentiae?
*** This is a forwarded message. Please reply to tg@cri.co.za
To: acarology@nhm.ac.uk
From: tg@cri.co.za
Subject: Rearing Neoseiulus barkeri on Tyrophagus putrescentiae?
Date: Thu, 22 Mar 2001 10:26:31 +0200
Greetings all
I am aware that Neoseiulus barkeri has lost popularity over the last
10
years but I would like to establish a culture of an indigenous strain
in
South Africa. Can anyone tell me whether it can be reared successfully
on
Tyrophagus putrescentiae? Thank you.
Tim
Tim G Grout, PhD
Research Manager: IPM
Outspan Citrus Centre
P O Box 28, Nelspruit, 1200 South Africa
Phone +27 13 759 8048 Fax +27 13 755 2281
www.citrusres.com
Email address: tg@cri.co.za
From: "Xavier Ducarme" <<Ducarme@ecol.ucl.ac.be>
To: acarology@nhm.ac.uk
Subject: Riccardoella
Date sent: Wed, 28 Mar 2001 14:19:49 +0200
Dear Xavier,
<italic>Riccardoella limacum</italic> and <italic>R.
oudemansi</italic> are recorded for the
first time from South Africa and I am busy preparing a short
publication on this. Both were apparently collected from the snail
<italic>Helix aspersa, </italic>however, I have not yet mounted
the few specimens
collected from a slug species. I can send you slide-mounted
specimens of <italic>R. limacum</italic> which, appear to be
the dominant
species and alcohol material of the same species if you are
interested. All my friends and colleagues are on the look-out for all
kinds of snails and slugs at the moment and I hope to even found
new species in future.
Kind regards
Eddie A. Ueckermann
Dear colleagues,
H. André and I are (re)describing some (new) species of
Riccardoella
(Actinedida: Ereynetidae). We will be delighted to receive additional
material for study:
- Riccardoella from Europe (NOT from snail and slugs)
- Riccardoella from other parts of the world (any habitat/host)
With many thanks for your courtesy,
*********************************************
Xavier DUCARME
Aspirant FNRS
Unité d'Écologie et de Biogéographie
Université catholique de Louvain
Place Croix du Sud 5
B-1348 Louvain-la-Neuve
+32-10-47 36 88
Fax: +32-10-47 34 90
Ducarme@ecol.ucl.ac.be
**********************************************
<color><param>0100,0100,0100</param>------- End of forwarded message -------
<nofill>
ARC-Plant Protection Research Institute
Biosystematic Division: Arachnology
Private bag X134
Pretoria
0001 South Africa
Tel:+27-12-329 3269-77 ext. 221
Fax:+27-12-329 3278
E-mail:rieteau@plant2.agric.za
Web:www.arc.agric.za
Dear colleagues,
H. André and I are (re)describing some (new) species of Riccardoella
(Actinedida: Ereynetidae). We will be delighted to receive additional
material for study:
- Riccardoella from Europe (NOT from snail and slugs)
- Riccardoella from other parts of the world (any habitat/host)
With many thanks for your courtesy,
*********************************************
Xavier DUCARME
Aspirant FNRS
Unité d'Écologie et de Biogéographie
Université catholique de Louvain
Place Croix du Sud 5
B-1348 Louvain-la-Neuve
+32-10-47 36 88
Fax: +32-10-47 34 90
Ducarme@ecol.ucl.ac.be
**********************************************
Greetings all
I am aware that Neoseiulus barkeri has lost popularity over the last
10
years but I would like to establish a culture of an indigenous strain
in
South Africa. Can anyone tell me whether it can be reared successfully
on
Tyrophagus putrescentiae? Thank you.
Tim
Tim G Grout, PhD
Research Manager: IPM
Outspan Citrus Centre
P O Box 28, Nelspruit, 1200 South Africa
Phone +27 13 759 8048 Fax +27 13 755 2281
www.citrusres.com
Email address: tg@cri.co.za
Dear Colleagues: I am working in artificial feeding of ticks through
membranes. I am interested in papers relatives to body weight changes
of
ticks during feeding. I would like to receive the article: Kitaoka
y Yajima,
1958; Physiological and ecological studies on some ticks. I. Process
of
growth by bloodsucking. Bull. Nat. Inst. Hlth., Tokyo (34): 135-47
and other
on the same or related subject. Thanks in advance. Rafael.
At 7:28 PM -0500 3/20/01, bhebert wrote:
>I have an infestation of mites in a beetle culture that are causing
some
>problems in the areas where I raise my animals. These may simply
be some
>sort of Dermatophagoides, but I have had tremendous infestations (over
a
>full cup) in some of my cultures and they are difficult to get rid
of.
>
>Would anyone care to give me a positive ID?
>
Several species of Acaridae and Histiostomatidae often overrun insect
cultures, especially if they're moist. Acarids in the genera
Tyrophagus
and Sancassania (=Caloglyphus) are the most common, but if there is
wet
material around, you can also get species of Histiostoma
(Histiostomatidae). It would be unlikely that Dermatophagoides
would be
involved in this situation since they feed primarily on high protein
materials. You didn't mention what kind of beetles you are rearing,
and
this could have some bearing on the mite associations.
All the best! - Barry
-------------------------------------------------------------------------
So many mites, so little time!
-------------------------------------------------------------------------
Barry M. OConnor
Professor & Curator
phone: (734) 763-4354
Museum of Zoology
FAX: (734) 763-4080
University of Michigan
e-mail: bmoc@umich.edu
Ann Arbor, MI 48109-1079 USA
Prezado Senhor,
consulte:
Jeppson, L.R., Keifer, H.H. & E.W. Baker, 1975
Mites Injurious to Economic Plants
Univ.California Press, 614 p + 74 pl.
ISBN 0 520 02381 1
Atenciosamente
Carlos Flechtmann
Universidade de Sao Paulo
Brasil
Dear experts,
I would very much appreciate information on the following specires:
Galendromus (Typhlodromus) occidentalis (Nesbitt)
Eotetranychus sexmaculatus (Riley)
I need adult body size, reproduction rate, and average life span, if
these
are available.
Many thanks,
Michael Fuller
----------------------------------------
Michael Fuller
Graduate Student
Department of Biology
University of New Mexico
Albuquerque, NM 87131
Dear members of the acarology list,
A couple of weeks ago, I posted a message asking for info about bird
mites
because my wife and I were suffering from what we thought were bird
mites.
An entomologist has since discovered that what I have on my skin are
baker's itch mites (rather than bird mites). Can someone tell me more
about
baker's itch mites (acarus siro)?
What ways are there to get rid of them from my environment? How can
I get
them off of my skin and out of my scalp?
Would home fumigation with Vikane kill this type of mite?
In a home, where do they live? where do they breed?
Do they breed on humans? lay eggs in/on the skin? in the scalp?
If I slap my skin with a piece of tape where I feel an itch, I often
get a
black speck on the tape. What is that black speck?
Are baker's itch mites contagious? Do I need to worry about giving them
to
other people via shaking their hands or kissing them on the cheek?
Will
other people get them if they come into our home, say for an evening
meal?
I would appreciate any info about these mites.
Ken Cook
Dear acarologists,
The Proceedings of the 10th International Congress of Acarology (Canberra)
is now in the hands of the publisher for final printing. We expect
that
publication will be in June/July. Everyone who paid a full registration
fee
will receive a free copy, and others can be bought from the publisher,
CSIRO
Publishing, Melbourne. I will advise again when more details are available.
Thank you all for your patience.
Bruce Halliday
***********************************************************
Dr. R. B. Halliday
CSIRO Entomology
GPO Box 1700
Canberra ACT 2601
Australia
Telephone (02) 6246 4085
Mobile 0438 543509
International Telephone (61) (2) 6246 4085
Fax (02) 6246 4000
International Fax (61) (2) 6246 4000
E-mail bruceh@ento.csiro.au
http://www.ento.csiro.au/research/natres/natres.html
***********************************************************
Dear fellow Acarologists,
Can anyone provide me the e mail ID / complete =
postal Address of the following acarologists
1) Dr N N Kuznetzov of USSR
2) Dr W. Calvin Welbourn of USA
3) Dr Andrzej Kazmierski of Poland
Thanks
Dr MS Dhooria
Senior Entomologist=20
Deptt. of Entomology
Punjab Agricultural University
Ludhiana-141004
Punjab
INDIA
Dear Soenke
We have a publication from our former collaborator Karl Dorn which deals
with Pyemotes:
Martin Grob, Karl Dorn, Stephan Lautenschlager; Getreidekrätze
-Eine kleine
Epidemie durch Pyemotes spezies; Der Hautarzt, 1998, 49: 838-843 (Springer
Verlag 1998). In the publication there are two pictures of Pyemotes.
One is
a hungry female, the other a fed female with the "Kugelbauch) and a
larva.
We have these pictures as slides. We could scan them and send them
by e-mail
if you are interested. Let us know. Thanks
Marcus
Marcus Schmidt
Beratungsstelle Schädlingsbekämpfung
Umwelt- und Gesundheitsschutz Zürich (UGZ)
Walchestrasse 33
Postfach
8035 Zürich
Tel.: 01 216 28 38
Fax: 01 216 50 41
e-mail: marcus.schmidt@gud.stzh.ch
http://www.ugz.stzh.ch.
> http://www.stadt-zuerich.ch/ugz/umwelt/index.htm
Dear Soenke
Take a look at
International Journal of Acarology
1987 - 13 (3) - front cover
There are several Acarologist in Germany who migh have this
periodical
C. Decke - decke@uni-duesseldorf.de
R. Gerecke - gerecke@uni-tuebingen.de
M. Maraun - maraun@bio.tu-darmstadt.de
Sincerely
Carlos Flechtmann
Univ. Sao Paulo
Brasil
***This is a forwarded message. Please reply to a.dernburg@vet-lyon.fr
______________________________________________________
Mar 2001 11:12:27 +0100
Date: Thu, 15 Mar 2001 11:15:44 +0100
To: acarology@nhm.ac.uk=20
From: Ann DERNBURG <a.dernburg@vet-lyon.fr>
I am seeking information on the economic impact of poultry mites
(Dermanyssus and Ornithonyssus) worlwide. I have DeVeneys articles.
Does
anybody have other info?
Thanks in advance,
Ann
Ann Dernburg
Ma=3DEEtre de Conf=3DE8rences=3D20
Unit=3DE9 de Ethnologie, Zootechnie, et Economie Rurale
Ecole Nationale V=3DE9t=3DE9rinaire de Lyon
1 Ave. Bourgelat
B.P. 83
69280 Marcy l'Etoile
tel: 04 78 87 25 25 poste 3380
fax/s=3DE9cr=3DE9tariat: 04 78 87 26 67
****This is a forwarded message. Please reply to soenke.mones@dva.de
From: Soenke Mones <soenke.mones@dva.de>
To: <ZhangZ@landcare.cri.nz>
Date: 3/16/01 7:07
Subject: Picture Request
Dear Zhi-Qiang Zhang,
My name is Soenke Mones, I am picture researcher at German science
magazine bild der wissenschaft. I am looking for a photo of Pyemotes
(hay mites). It is in connection with an article about Mad Cow Disease.
Do you know a scientist I could ask about this photo?
I would be very grateful if you could help me.
Yours sincerely,
Soenke Mones
--
Soenke Mones
Deutsche Verlags-Anstalt GmbH
Fotoredaktion bild der wissenschaft
Neckarstr. 121 - D-70190 Stuttgart
Telefon +49 711 22292712 Telefax -750
Zhi-Qiang Zhang
Acarologist
Landcare Research
P.B. 92170
Auckland
New Zealand
phone [0064-9] 815-4200 ext 7069
Fax (0064-9) 849-7093
E-mail zhangz@landcare.cri.nz
webpage www.nhm.ac.uk/hosted_sites/acarology/zhang/
Dear Colleagues,
Do all larval mites of superfamily Tetranychoidea have "duplex
setae"?
Have you ever seen the structure in other families of Prostigmata?
Qinghai Fan
-------------------------------------------------------------------
Dr. Qinghai Fan
Department of Plant Protection
Fujian Agricultural and Forestry University
Fuzhou 350002, Fujian
P.R. China
phone: 86 591 3771181
email: qhfan@pub2.fz.fj.cn
http://www.nhm.ac.uk/hosted_sites/acarology/saas/member/Fanqh.htm
Dear acarologists:
Can anyone help me with this publication:
Gimenes, D.F., 1964. Staining rickettsiae in yolk-sac cultures. Stain Technol., 39: 135-140.
I am working with rickettsias in ticks and I need this publication for
my protocol o if is possible send me an abstract of the method.
I would like to have a fotocopy of that paper, please send me any help to CASILLA O3 ICA-PERU, SUDAMERICA or Lemurica@latinmail.com
Thank you in advance
Leonardo Mendoza Uribe
División de Entomología
Instituto Nacional de Salud
Lima, Perú
_________________________________________________________
http://www.latinmail.com. Gratuito, latino y en español.
> Does anyone know of references/info sources on microsporidia that
infect
> mites? Some of my stock cultures have suddenly lost fertility
and a
> colleague who works on insect epidemiology suggests that microsporidia
may
> be the cause.
I know only oribatid mites......
Purrini, K. (1981) Studies on some amoebae (Amoebida) and Helicosporidium
parasiticum
(Helicosporida) Infecting moss-mites (Oribatei, Acarina) in dorest
soil samples.
Arch. Protistenk. 124: 303-311.
Purrini, K., Bukva, V. and Baumler, W. (1979) Sporozoen in Hornmilben
(Oribatei, Acarina) aus Waldboden Suddeutschlands nebst
Beschreiburng
von Gregarina postneri n. sp. und Gregarina fuscozetis n. sp. (Gregarinidae,
Sporozoa,
Protozoa). Pedobiologia 18: 329-339.
Dr. Purrini reported many cases of Sporozoa in Orimbatid mites.
Best wishes
Satoshi
@@@@@……@@@@@@@@@@@@@@@@@@@@@@@@……@@@@@
Satoshi Shimano, Ph. D.
Department of Upland Farming
Tohoku National Agricultural Experiment Station
Arai, Fukushima, 960-2156, Japan
satoshis@fk.affrc.go.jp
Fax. +81-24-593-2155
>Does anyone know of references/info sources on microsporidia that
>infect mites?
Bjornson S. and Keddie B. A. 1999. Effects of Microsporidium
phytoseiuli (Microsporidia) on the performance of the predatory mite,
Phytoseiulus persimilis (Acari: Phytoseiidae). Biological Control 15:
153-161.
Bjornson S. and Keddie B. A. 2000. Development and pathology of two
undescribed species of microsporidia infecting the predatory mite,
Phytoseiulus persimilis Athias-Henriot. Journal of Invertebrate
Pathology 76: 293-300.
Bjornson S., Steiner M. Y. and Keddie B. A. 1996. Ultrastructure and
pathology of Microsporidium phytoseiuli n sp infecting the predatory
mite, phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae).
Journal of Invertebrate Pathology 68: 223-230.
Bjornson S., Steiner M. Y. and Keddie B. A. 1997. Birefringent
crystals and abdominal discoloration in the predatory mite
Phytoseiulus persimilis (Acari: Phytoseiidae). Journal of
Invertebrate Pathology 69: 85-91.
Larsson J. I. R., Steiner M. Y. and Bjornson S. 1997. Intexta
acarivora gen. et sp. n. (Microspora: Chytridiopsidae):
ultrastructural study and description of a new microsporidian
parasite of the forage mite Tyrophagus putrescentiae (Acari:
Acaridae). Acta Protozoologica 36: 295-304.
Ribeiro M. F. B. and Guimaraes A. M. 1998. Encephalitozoon-like
microsporidia in the ticks Amblyomma cajennense and Anocentor nitens
(Acari : Ixodidae). Journal of Medical Entomology 35: 1029-1033.
Van der Geest L. P. S., Elliot S. L., Breeuwer J. A. J. and Beerling,
E. A. M. 2000. Diseases of mites. Experimental and Applied Acarology
24: 497-560.
Weiser J., Rehacek J., Zizka Z., Ciampor F. and Kocianova, E. 1999.
Nosema slovaca Weiser et Rehacek, 1975 and Unikaryon ixodis (Weiser,
1957) comb. n. in ixodid ticks. Acta Parasitologica 44: 99-107.
Regards,
Dr. Robert Cruickshank
Division of Environmental and Evolutionary Biology (DEEB)
Institute of Biomedical and Life Sciences (IBLS)
University of Glasgow
Graham Kerr Building
GLASGOW G12 8QQ
Strathclyde
Scotland, U.K.
Telephone +44 (0)141 330 6626 (office)
+44 (0)141 330 3561 (lab)
Fax
+44 (0)141 330 2792
e-mail rhc3d@udcf.gla.ac.uk
www http://taxonomy.zoology.gla.ac.uk/~rcruicks/rcruickshank.html
Hello everyone
Does anyone know of references/info sources on microsporidia that infect
mites? Some of my stock cultures have suddenly lost fertility
and a
colleague who works on insect epidemiology suggests that microsporidia
may
be the cause.
Many thanks
Tim
Dr Tim Benton
Institute of Biological Sciences
University of Stirling
STIRLING
FK9 4LA, UK
tel (0)1786 467809
fax (0)1786 464994
email t.g.benton@stir.ac.uk
I am looking for some sort of adhesive that might be effectively used
to trap mites
that migrate into it.
Specifically, the mite of interest is clover mite, Bryobia praetiosa.
It readily
will walk up stakes placed in turfgrass areas but I would like some
sort of adhesive
that would trap in place those that wander onto the stakes. (These
mites, as most
of you probably know, readily drop when disturbed, making quanitification
of their
presence difficult.)
I have tried the standard materials I use for insects, Tanglefoot and
Stick-em
Special (USA product names). The mites, upon contact, avoid the
treated surface and
do not get ensnared.
Any suggestions as to an adhesive that is good for coating a trap that
would capture
and hold a clover mite that contacts and subsequently walks across
the surface?
Whitney Cranshaw
Colorado State University
Ft. Collins, CO USA
wcransha@ceres.agsci.colostate.edu
Dear Acarologists,
some time ago I informed that I was trying to get information on salivary
composition of eriophyoid mites.
I have preliminary tested about a dozen of species causing several injuries
to get salivary spit in oil. And I tested several oils finding that
the
mites are able to spit largely in oil commonly used for immersion oil
objectives.
The species which gave us more success was Aceria caulobia, common in
Southern Italy on Suaeda fruticosa, causing large galls on the stems.
By means of several cares we were able to concentrate (by means of an
air
flow) a lot of specimens with the oil and then carefully we sieved
the
fluid (oil and phosphate buffer) by a nylon filter with pore less than
20
microns to avoid the passage of individuals into the filtered emulsion.
We
checked also that the specimens were integre after the extraction.
Than we tested the water phase with two bio-tests:
1) wheat coleoptile length for auxine presence with three different
thesis:
phosphate buffer, extracted water phase, and water solution of pure
hormon
(alfa indol acetic acid)
2) radish cotyledon weight for kinetin presence with three different
thesis: phosphate buffer, extracted water phase, and water solution
of pure
hormon (cinetine)
The preliminary tests gave us interesting answers. Of course we have
to
re-arrange the amount of the hormones, but the cotyledons and the
coleoptiles in extracted water phase increased largely more than the
phosphate buffer and a bit less than the hormon solutions.
But we would like to go over this. Of course we have to repeat the
bio-tests, but we would like to try in defining the chemical composition
by
other techniques.
So, the question is: are there people which have been worked with CG-MS
or
HPLC for getting these data? How protocol they have applied?
Moreover, one hypothesis is that eriophyoids could transmit small peaces
of
nucleic acid by saliva into the plant cells. So, the question is: are
there
people which have been worked on this way? Could they give me information?
PCR could work in this, but .... ?
Finally, I have tried several times to find the chemical composition
of the
Olympus immersion oil for ordinary use in microscopy, but I was not
able to
get data. Could someone help me?
Sorry for this long letter, but the topic is so complicated that I am
not
able to solve by myself all the troubles.
Thanks a lot
Enrico de Lillo
CC: <delillo@agr.uniba.it>
Date: Fri, 2 Mar 2001 09:20:01 -0800
To: acarology@nhm.ac.uk
From: "Andrew J. Bohonak" <bohonak@sciences.sdsu.edu>
Subject: Re: extracting DNA from mites
Hello all,
just to add another 2 cents...
I have been sequencing water mites and other minute freshwater
arthropods (admittedly, Hydracarina are larger than many other mite
taxa) after extracting with 1) standard phenol-choroform, 2) chelex
and 3) Qiagen DNEasy kits.
I would endorse the Qiagen kits and chelex for ease of use and
suggest that overnight incubation in the lysis buffer is preferable
to incubation for only a few hours. However, quality of DNA in
chelex seems to degrade over time, whereas the Qiagen extractions do
not. It may have something to do with the buffers.
Andy
--
Andrew J. Bohonak
Assistant Professor
San Diego State University
Department of Biology
San Diego, CA 92182-4614
= = = = = = = = = = = = = = = = =
Phone: 619-594-0414
Fax: 619-594-5676
Email: bohonak@sciences.sdsu.edu
Web: http://www.bio.sdsu.edu/pub/andy/index.html
Office: 212 Life Science North
Hello Lucie (and other acarologists),
I would recommend you try the DNAeasy tissue kit from QIAGEN (no
affiliation). Recently I have been using this kit to extract DNA from
lice while preserving the exoskeletons for slide mounting as voucher
specimens. First I decapitate the louse, then I put both the head and
body (with no further grinding necessary) into the lysis buffer
provided in the kit and incubate at 55 degrees C over two nights. I
then remove the head and body of the louse, which are still intact,
and these are subsequently slide mounted as voucher specimens. These
vouchers can be used to check the identification of the lice I have
sequenced, or to score morphological characters for use in
phylogenetic analyses along side the molecular characters. DNA is
extracted from the remaining lysate according to the protocol given
in the QIAGEN handbook. I've also used this protocol on mites and
complete grinding up is only necessary for the very smallest mites.
This is far and away the best method I've found for extracting DNA
from microarthropods and I urge you to try it.
For more information, check out these websites...
http://www.qiagen.com/catalog/chapter_05/chap5a1.asp
http://www.qiagen.com/literature/handbooks/dny/dnytiss/1011607_dnsy_499.pdf
For other methods of extracting DNA from mites check out the
protocols on my website at
http://taxonomy.zoology.gla.ac.uk/~rcruicks/pb.html.
Good Luck!
Dr. Robert Cruickshank
Division of Environmental and Evolutionary Biology (DEEB)
Institute of Biomedical and Life Sciences (IBLS)
University of Glasgow
Graham Kerr Building
GLASGOW G12 8QQ
Strathclyde
Scotland, U.K.
Telephone +44 (0)141 330 6626 (office)
+44 (0)141 330 3561 (lab)
Fax
+44 (0)141 330 2792
e-mail rhc3d@udcf.gla.ac.uk
www http://taxonomy.zoology.gla.ac.uk/~rcruicks/rcruickshank.html
Dear acarophiles,
I have just found that the message that I sent to acarology list has
been
bounced. I am, accordingly, sending it off again. I just thought that
this
year's ionosphere is thick enough to propagate this signal up there...
Best, Cheol-Min
Dear Lucie and other acarophiles,
Hybrid but solid state acarologists, who study both morphology and
molecules, at the OSAL here, led by Hans Klompen, follow *standard*
*homebrewed* CTAB extraction protocol with 1-6 individuals of mites,
which
works *very* fine with freshly preserved materials. Fresh preservation
implies that the mites are, if parasitic or phoretic, separated from
their
hosts and then preserved in at least 95% ethanol. These samples are
then
stored at -80C so-low freezer until they are materialized.
I think that the closest person to be contacted from the UK regarding
this
matter is B. Fenton at Scottish Crop Research Institute, who extracted,
PCRed, and RFLPed DNA from eriophyid mites. He used Nucleon Kit (Scotlab,
Lanark, Scotland). His address is:
B. Fenton
Scottish Crop Research Institute
Invergowrie, Dundee DD2 5DA
Scotland, U.K.
All the best, Cheol-Min
On Thu, 1 Mar 2001 17:26:45 UTC Lucinda Evans wrote,
>Hello All,
>
>Can anyone suggest a useful protocol for extracting DNA from very
samll
>numbers of mites ??????
>
>
>I am running molecular work on Psoroptes and Sarcoptes mites and am
having
>difficulties getting useful DNA out of fewer than 20 or so pooled
individuals.
>
>Any help anyone can send my way will be gratefully received.
>
>Thank you in advance
>Lucie Evans
>
>Hatherly Labs
>University of Exeter
>Prince of Wales Road
>Exeter, EX4 4PS, UK
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Nature created species; Man created genera.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Cheol-Min Kim
Acarology Laboratory
Museum of Biological Diversity
The Ohio State University
1315 Kinnear Rd.
Columbus, OH, 43212-1192, U.S.A.
Tel: +1-(614) 292-7180
Fax: +1-(614) 292-7774
E-mail: Kim.296@osu.edu
CC: <acarology@nhm.ac.uk>
Lucie (and anyone else who is interested),
I have had good luck extracting DNA from individual larval Dermacentor
ticks
(pretty small, but maybe not as small as mites?) using the IsoQuick
Nucleic
Acid Extraction kit (ORCA Research, Inc., Bothell Washington, 98041,
USA).
I've modified the protocol to include grinding in LN2 and Proteinase
K
digestion before extraction. I resuspend the DNA in 50 ul of water
and have
plenty of DNA for 20-30 PCR reactions. For small mites you may want
to
resuspend the DNA in a smaller volume of water. I've done about 100
larvae
from 5 different species and had 100% success amplifying 16s rDNA
and ITS2.
Send me an E-mail if you would like more details.
Glen
********************************
Glen A. Scoles, Ph.D.
Research Entomologist
USDA, ARS, Animal Disease Research Unit
4015 ADBF, Washington State University
PO Box 646630
Pullman, WA, 99164-6630
Office: (509) 335-6337
Lab: (509) 335-6347
FAX: (509) 335-8328
E-mail: scoles@vetmed.wsu.edu
********************************
> ----------
> From: Lucinda Evans
> Sent: Thursday, March 1, 2001 9:26 AM
> To: acarology@nhm.ac.uk
> Subject: extracting DNA from mites
>
> Hello All,
>
> Can anyone suggest a useful protocol for extracting DNA from very
samll
> numbers of mites ??????
>
>
> I am running molecular work on Psoroptes and Sarcoptes mites and
am having
>
> difficulties getting useful DNA out of fewer than 20 or so pooled
> individuals.
>
> Any help anyone can send my way will be gratefully received.
>
> Thank you in advance
> Lucie Evans
>
> Hatherly Labs
> University of Exeter
> Prince of Wales Road
> Exeter, EX4 4PS, UK
Hi Lucie,
We've had fair luck using a standard Chelex DNA extraction technique*
using
batches of 5 mites (usually gravid females), but even a single mite
or mite
eggs will work. The specimens are mostly from cultures
(Mesostigmata) or
field collections (Tetranychoidea) and were killed in 100% ethanol
and then
stored in a freezer, or snap frozen at -70. We've had less
luck with
older specimens from 80% ethanol, room temperature stored collections.
Dr Nick Campbell (N.Campbell@mailbox.uq.oz.au) might be able to give
you
some more technical advice.
*Hillis, D.M., Mable, B.K., Larson, A., Davis, S.K., and Zimmer, E.A.
1996. Nucleic acids IV: sequencing and cloning. In "Molecular
Systematics"
(D. M. Hillis, C. Moritz and B. K. Mable, eds), pp 321 - 384.
Sinauer,
Sunderland, Massachusetts.
Cheers,
Dave Walter
>Hello All,
>
>Can anyone suggest a useful protocol for extracting DNA from very
samll
>numbers of mites ??????
>
>
>I am running molecular work on Psoroptes and Sarcoptes mites and am
having
>difficulties getting useful DNA out of fewer than 20 or so pooled
individuals.
>
>Any help anyone can send my way will be gratefully received.
>
>Thank you in advance
>Lucie Evans
>
>Hatherly Labs
>University of Exeter
>Prince of Wales Road
>Exeter, EX4 4PS, UK
Dr David Evans Walter
Department of Zoology & ENTOMOLOGY
Hartley-Teakle Building
The University of Queensland
St Lucia, QLD 4072 Australia
phone: 07-3365-1564
fax: (61) 7-3365-1922
The University of Queensland Program in Entomology
http://www.uq.edu.au/entomology/courses.html
Cooperative Reseach Centre for Tropical Plant Protection
http://www.tpp.uq.edu.au/
Visit the Mite Image Gallery at:
http://www.uq.edu.au/entomology/mite/mitetxt.html
Acarina Collection:
http://www.uq.edu.au/entomology/museum/mites/miteord.html
Beta test the LucID keys to:
Families of Parasitiformes in Soil:
http://www.lucidcentral.com/keys/cpitt/public/Mites/Parasitiformes/Default.htm
Soil Microarthropods
http://www.lucidcentral.com/keys/cpitt/public/Mites/Microarthropods/Index.htm
Orders, Suborders and Cohorts of Mites in Soil
http://www.lucidcentral.com/keys/cpitt/public/Mites/Soil%20Mites/Index.htm
CC: <acarology@nhm.ac.uk>, <N.Campbell@mailbox.uq.oz.au>
Hello All,
Can anyone suggest a useful protocol for extracting DNA from very samll
numbers of mites ??????
I am running molecular work on Psoroptes and Sarcoptes mites and am
having
difficulties getting useful DNA out of fewer than 20 or so pooled individuals.
Any help anyone can send my way will be gratefully received.
Thank you in advance
Lucie Evans
Hatherly Labs
University of Exeter
Prince of Wales Road
Exeter, EX4 4PS, UK