ISSN 1362-1971
An international journal of the Systematic and Applied Acarology Society, published since 1996
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ISSN 1362-1971 An international journal of the Systematic and Applied Acarology Society, published since 1996 [Aims] [Editors] [Content] [Subscriptions] [Contact details] [Society Homepage] |
Systematic & Applied Acarology (2002) 7, 3-14Molecular markers for the phylogenetics of mites and ticks
ROBERT H. CRUICKSHANK
Ecology and Entomology Group, Soil, Plant and Ecological Sciences Division, Lincoln University, PO Box 84, Lincoln,
Canterbury, New Zealand; cruicksr@lincoln.ac.nz
Abstract
Molecular methods are becoming increasingly important in systematic acarology. In this review I describe the
properties of the ideal molecular marker and compare these with genes that have been used for phylogenetic
studies of mites and ticks. The second internal transcribed spacer of the nuclear ribosomal gene cluster (ITS2)
and the mitochondrial protein-coding gene cytochrome oxidase I (COI) together provide a powerful tool for
phylogenetics at low taxonomic levels. The nuclear ribosomal genes 18S and 28S rDNA are equally powerful
tool for phylogenetics at the deepest levels within the Acari. What appear to be lacking are markers that are
useful at intermediate levels. The mitochondrial ribosomal genes 12S and 16S rDNA have not lived up to their
initial promise. I suggest some nuclear protein-coding genes that may be suitable alternatives. Methods for
collection and storage of mites for molecular work, DNA extraction and phylogenetic analysis are also briefly
discussed.
Key words: Acari, molecular systematics, phylogenetics
Copyright 1999-2002 Systematic and Applied Acarology Society
Last updated 15 Jul. 2002
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