The FEI Quanta 650 FEG scanning electron microscope (SEM) is used for high-resolution imaging and semi-quantitative X-ray microanalysis of both conductive and non-conductive specimens at nanometer resolution (magnification range from 5-1,000,000x).
The FEI Quanta 650 FEG has two modes:
- Variable pressure (VP) mode (pressure range 100-200Pa)
Used for high-resolution imaging of non-conductive samples in both secondary electron and backscatter modes.
- High-vacuum (HV) mode (pressure range 10-2-10-4 Pa)
Used for high-resolution imaging of conductive samples in both secondary electron (SE) and backscattered (BSE) modes.
It is equipped with the following accessories and features:
- a low-vacuum cone, mounted on the final lens, for low-kV imaging under VP conditions to improve imaging of surfaces, with reduced beam penetration
- a beam sleeve, mounted on the final lens, to improve the performance of Energy Dispersive X-ray micro-analysis (EDX) in VP conditions by reducing beam skirt
- beam-deceleration (BD) mode for ultra-high resolution backscatter imaging of flat samples under HV conditions
- drift-correction mode for imaging highly charging samples
- MAPS software for tiling and creating large montage images at high resolution, which allows for automated image collection of large areas or multiple areas
- custom-built vacuum pumping system allowing for slow and fully controllable pump down time for specimens sensitive to rapid decompression rates
- additional micro tilt/rotate stage (Deben) for high-precision work on small, demanding samples, e.g. pinned insects
- Bruker Flat Quad 5060F energy dispersive X-ray detector (EDS) for ultra-fast and shadow-free semi-quantitative elemental analysis and hyperspectral mapping, even on samples that are non-conductive and highly topographic
- large area elemental mapping of fossilised arthropods
- high-resolution backscattered (BSE) imaging of very fine scale mineral particles
Technique: high-resolution and variable pressure scanning electron microscopy (SEM)
Modes: variable pressure (VP) mode (pressure range 10-200Pa) and high vacuum (HV) mode (pressure range 10-2-10-4Pa)
- conventional secondary electron detector (ETD) for high-vacuum imaging
- directional/concentric backscattered electron detector (BSD) used for high-resolution imaging in both high vacuum (HV) and variable pressure (VP) modes
- variable pressure secondary electron detector (LFD) for high-resolution low-vacuum imaging