Molecular diagnostics for species identification and evolutionary analysis
A one-week introductory course about molecular phylogenetic analyses.
Designed for taxonomists and ecologists at faculty, post-doc and post-graduate level, the course is taught by Museum scientists.
Taught by Museum scientists, the course will benefit taxonomists and ecologists wishing to gain expertise in molecular diagnostics and phylogenetic analysis for systematic, taxonomic and ecological studies. Please note that this course assumes no prior experience with DNA sequence analyses.
The course will teach participants the steps involved in producing molecular phylogenies from raw Sanger and mitogenomic next-generation sequence data (Illumina paired-end). Lectures will provide theoretical background prior to any practical applications.
Each step, involving specific software, will be demonstrated and practiced in hands-on computer lab sessions. During computer lab sessions, we aim to have three tutors present to ensure a high level of assistance for the 14 participants.
On the last day, participants will be able to analyse their own data or return to sections of the course of their own choice.
Course participants will receive theoretical and practical training on how to:
- Edit raw Sanger sequence data for phylogenetic analysis (edit chromatograms, identify contamination, align sequences, remove ambiguously aligned sites)
- Use BLAST to find sequences homologous to a query sequence using GenBank
- Perform Bayesian/maximum likelihood analyses using appropriate models
- Interpret tree topologies and nodal support
- Produce publication-ready trees Upload new sequences onto GenBank Assemble and annotate NGS data
Participants will also
- Learn about different methods for phylogenetic analysis
- Understand some problems associated with species delimitation
- Recognise the potential of next generation sequencing
- Recognise the pros and cons of different markers for systematics and barcoding
Dates and times
A five day course:
20-24 Februrary 2017
Closing date: 1 December 2016
How to apply
The course is available to taxonomists and ecologists at faculty, post-doc and post-graduate level. There are 14 spaces available, and priority will be given to those with NERC funding.
Download and complete the application form (PDF 92KB) and return it to the address on the form by the closing date 1 December 2016.