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3 Posts tagged with the non_marine tag
1

Before I tell you about our time in the lab, I thought I would share with you another video taken by Paul Pearce Kelly in Cuc-Phuong. The video below is of a predatory slug species from the genus Atopos. These slugs either eat small microsnails whole or bore through larger snails' shells using their toothed, tongue-like radula.

 

 

 

After our exciting and productive time in Cuc Phuong National Park we headed back to Hanoi to process the material for frozen tissue collections. Our expedition has been jointly funded by the Natural History Museum and the Frozen Ark, and one of our aims is to collect frozen tissue material and viable cells.

 

The Frozen Ark was set up in 1996 and is run by a consortium of 22 universities, museums, zoos and aquaria from 8 different countries, with the aim to conserve frozen cells containing DNA from endangered animals before they go extinct. Since many limestone habitats are under threat from the construction industry (the limestone is used in the manufacture of cement and other building materials), we decided that terrestrial molluscs would be a great addition to the Frozen Ark collections.

 

chris and jackie.JPG

Chris preparing tissue whilst Jackie puts the samples on ice.

 

The specimens used for these processess have been brought back from Cuc-Phuong live, to be processed in the laboratory. For the frozen tissue samples, small slices of the foot of the snail (the part it moves on) are placed in sterile tubes and frozen at -80 degrees Celsius. These samples will hopefully yield high quality DNA. Five multiple sets are stored for our current research, long term Museum storage, the Frozen Ark in London, the Frozen Ark in Nottingham and for the Frozen Ark in Vietnam.

 

The viable cell preparations are also taken from tissue samples but they are mashed up in an antibiotic solution (to prevent damage by bacteria etc.), and then mixed with a buffer chemical so that as the tissues are frozen, again at -80 degres Celcius, the cells will not fracture and will remain whole. It is hoped that future technology will allow us to use these cells and the genetic content within to re-create and re-introduce extinct species.

 

lab.JPG(L-R) Jackie, Chris and Fred hard at work on the cold-chain-gang (sorry terrible molecular joke).

 

The two experts in our group helping us with these processes are Jackie Mackenzie-Dodds and Dr. Chris Wade, who are both on the advisory board of the Frozen Ark. Jackie is the collections manager of the molecular and frozen tissue collections at the Museum, and Chris is a lecturer of genetics and a molecular biologist at Nottingham University. I'm glad they are both here as the frozen tissue preparations are complex and intricate and I have never done anything like this before.

 

We have spent the last few days collecting in Huu Lien Nature Reserve and tomorrow will be our last day collecting in the field so in my next blog I'll be telling you all about the strange and wonderful things we have found, and a little about our last few days in Hanoi.

 

Fruit for Thought

 

As well as the delicious savoury dishes we have been sampling a wealth of unusual (at least to me) fruit. My favourite has to be the Rambutan, a egg-sized red fruit covered in green hairs. I'm afraid I don't have any pictures because I ate them all before I thought to take a photo so you'll have to Google them. They taste a bit like a grape but are sweeter and juicier - delicious.

 

fruit.JPGPersimmon (the red fruit) and Guava's (the green ones) with a little lotus tea - what a lovely afternoon treat.

 

We've also been eating ...

 

Pomelo - a sharp citrus fruit with a grapefruit-like taste

Guava - like a tropical quince

Persimmon - a bit like across bewteen a plum and a tomato, much nicer than it sounds

Longans - like large lychees but sweeter

Forest Bananas - shorter but wider than our usual bananas, tastes earthier and banana-ery if that makes any sense

Jicama - a tuber that tastes like apple and is often served with savoury courses

 

Bite Me

 

0 Leech bites ... all though I have a funny feeling this may change tomorrow as it is due to be a stormy evening

12 mosquito bites

Ant bites, fading and almost gone

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We arrived in Cuc Phuong National Park on Friday afternoon. Set up in 1962, the park is the oldest in Vietnam and covers an area of over 22,200 ha. It is home to more than 2,000 species of trees, 110 species of reptiles and amphibians, 308 species of bird and 125 species of mammal, including the Clouded Leopard, Delacour's Langur, Owston's Civet and the Asian Black Bear.

 

Since the national park is so rich in snail diversity we could barely wait to start collecting and bagged our first few specimens as we excitedly stepped out of our van. After a hearty meal (more later) and a good night's sleep under our mosquito nets we were ready to head out into the field. Our first collecting site was a limestone outcrop, which also contains an archaeological cave where human tools and remains dating to over 7,500 years ago have been recovered.

 

Chasing Snails

 

Collecting snails is harder than it sounds, as we have to make sure we get specimens that live in soil and leaf litter, on the rocks and stones, in the trees and on vegetation. In the hot and humid conditions of the tropics this is difficult and sweaty work, but at least when we do find them they aren't too hard to catch.

instructer Frd.jpgFred Naggs (R) gives Hao Luongvan (L), Mr Kiem, our driver (C), and myself a quick Iecture.

 

On our first day we found an amazing variety of specimens, from microsnails less than 1mm tall that hide in the crevices of the limestone rock face, to larger and often strikingly coloured species. One of my favouites was the white form of Camaena gabriellae (pictured below left) which we found crawling on the bark of trees and managed to catch by knocking them with a stick in to a traditional conical Vietnamese hat, which did the job perfectly.

 

I am also particularly fond of the elongate group of snails called the Clausiliidae (pictured below right), which are found on limestone in large numbers, their bodies barely peaking beyond the shells as they graze on lichens and algae.

 

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Species found on day one include Camaena gabriellae (L) and Tropidauchenia sp. from the Clausillidae (R).

 

Today I would like to introduce you to another Vietnamese member of our team, Mr Hao Luongvan (pictured in first image). Hao works for the Forestry Commision and is based in Hoang Lien National Park, Sapa in northen Vietnam. He has been studying molluscs for the last ten years.

 

We first met Hao on our visit to Cuc Phuong in 2007 and have worked closely with him ever since. Not only does he a have an encyclopaedic knowledge of the variety of habitats, plants and animals across Vietnam, but he has been instrumental in helping us gain access to different national parks and make vital links with important government and conservation departments.

 

Turtle Power

 

After a hard day's work we managed to fit in a visit to the Cuc Phuong Turtle Sanctuary. The centre was set up in 1998 as a safe area to house and breed the 19 native species of Vietnames turtles (of which 3 are found in Cuc Phuong), as well as to increase public awareness of the threats poaching poses to these amazing creatures.

 

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Terrapin in Cuc Phuong Sanctuary. Poaching is the main source of their decline.

 

The centre was really inspiring, as to date over 900 turtles have been born there from animals confiscated from illegal traders. Plus we got to sit on a life-size model of the Yangtze giant softshell turtle (Rafetus swingoei), which can reach over a metre in length.

 

Specimen Sorting, Identification and Preservation

 

After our brief foray in to the world of chelonians it was time to get back to work. The first job is to sort all the snails into species based on shell and body shape, colour and sculpture (sculpture means the 3D surface - is it ridged, smooth or bumpy etc.). Once we have done this we choose one specimen to take a tissue sample from, and preserve this in 100% ethanol for molecular analysis.

 

snail-sorting.jpgSorting snails in the sunshine, it's a hard life ... honest.

 

We then split the remainder of the individuals into those to be preserved in 80% ethanol for anatomical analysis and those to be taken back to Hanoi alive for viable cell preservation (more on this in a future blog post). This is my favourite part of the collecting day as we get to compare what we have found and there is always something unusual or unexpected - this time we had both aplenty! On our first haul we could not believe the diversity and volume of species we managed to collect - this really is an amazing habitat for molluscs and even more exciting than usual as we think in our first day we not only have a new species or two but possibly even a new genus .... so watch this space.

 

Food For Thought

 

After a long but fruitful day it was time to retire for dinner and no blog post of mine would be complete without some mention of food. Tonight was the first time on this trip that I got to have some of my favourite Vietnamese food; a green vegetable called Morning Glory (rau muống in Vietnamese or Ipomoea aquatica in science speak). The spinach-like stems and leaves are fried in garlic and chilli and are bitter but wonderfully moreish. I ate more than I should have with fried chicken, sesame roasted pork and sumptuous sticky rice washed down with locally made Vietnamese brandy (don't ask!).

 

DSC_1016_small.JPGMorning Glory (Ipomoea aquatica) the green stuff on the left ... much nicer than it looks!

 

As I write this (in the courtyard of our accomodation with toads barking and cicadas chirping) I have just finished sorting out Sunday's specimen haul, also an impressive bounty, and I will be reporting back shortly on some new and exciting discoveries along with some of our further adventures in Cuc Phuong.

 

Biting animals update

 

As of Sunday evening (our time) I have;

0 leech bites

3 Mosquito Bites

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Today I am heading off on a plane to Vietnam. Not for a sumptuous seaside sojourn or a historic amble around Hanoi, no instead I will be on fieldwork collecting terrestrial slugs and snails in the national parks of Vietnam.


I'm Jon Ablett, curator of non-marine Mollusca and Cephalopoda at the Museum. I've been on collecting trips to Vietnam before in 2007, 2011 and 2012, but I'm really looking forward to heading back there not only for the wonderful food, landscape and people (and did I mention the food?), but also for the interesting slugs and snails that we hope to find.

Picture1_forblog.jpgCollecting snails in Vienam in 2011; definitely not a competition to see who has found the best specimen.

 

Why Vietnam? Well, Vietnam is an amazingly diverse country with a broad latitudinal range - from north to south it is over 1,000 miles long. It also has a wide altitudinal range, from over 3,000m in the Hoang Lien Mountains in northern Vietnam, to coastal lowlands and the Mekong Valley.

 

The Annamese Mountains, which run north to south between Vietnam, Laos and NE Cambodia, are the most extensive limestone hill ranges in Asia. Snails require calcium to form their shells, and as a rule snail diversity increases as the amount of calcium in the environment increases. The rich calcium limestone in these mountains provides incredibly diverse habitats for a highly diverse range of molluscan species that, until now, have barely been studied.

 

Why snails?


Apart from the fact that I am the curator in charge of the land snail (and cephalopod...) collections at the Museum, there are more recorded extinctions of land snails than for all other animal groups combined. This makes land snails a key group for recognising why and where extinctions are taking place. It also means that snails could be used to demonstrate the effectiveness of various conservation measures, by acting as sensitive indicators of environmental change.

 

Also, since the Museum has a large historical collection of slugs and snails from Vietnam, by collecting specimens over a variety of timescales we can look at changes within and between species.

Cyclophorus for blog.jpgCyclophorus sp. in leaf litter. Notice the trap door like operculum by the tail.


The aim of our work in Vietnam is to collect new terrestrial land snail material to enhance the collections of both the Natural History Museum and the Vietnam National Museum of Nature, to provide tissue samples for molecular work, and to test new collection and storage techniques for our frozen tissue collections.

 

My colleagues and co-conspirators for our fieldwork include Museum staff and students as well as scientists from Nottingham University, the Zoological Society of London, the Vietnam National Museum of Nature and the Vietnam National Park Service. I hope that throughout my time in Vietnam I can tell you a little bit about the work that will be done by me and the team, explain some of the collection and preservation techniques we use and also a little about the trials and tribulations, highlights (and lowlights?) that occur along the way.

 

I'm just off to finish up packing up my collection equipment, suncream and mosquito repellent and hopefully the next time I blog I will be settled in Hanoi with a few new stories to tell.