Arthropod specimens and genome skimming: Extracting a large panel of diet, symbiotic and phylogenomic information
Benjamin Linard, Department of Life Sciences, NHM
Wednesday 15 October 1100
Sir Neil Chalmers seminar room, Darwin Centre LG16 (below Attenborough studio)
Genome skimming (GS) is the shallow sequencing of the DNA extracted from pooled specimens. This approach was successfully tested on plants to extract simultaneously chloroplast / mitochondria / rRNAs and nuclear markers for phylogenomics and ecological studies. We previously produced insect specimen pools, initially to generate hundreds of complete mitochondria but also skimming the nuclear genomes of the specimens and their gut content. We will describe here the promising potential of GS when applied to arthropods.
In particular, we will show: (1) how trophic interaction between aphid preys, ladybirds (Coccinellini specimens) and associated symbiont can be skimmed from gut contents; (2) why a large panel of DNA markers (mitochondria, coding regions, repeats) are systematically leachable from insect pools through GS; (3) why applying GS to field collected material could extend our knowledge of insect genome evolution and uncover several ecological messages.
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