Jeremy, Thursday, October 30th, 2008
As we continue inexorably southward days are beginning to merge into each other and we are going a bit stir-crazy. So any excuse for a change of routine is very welcome and last week we hit the biggest one, crossing the equator.
This happened on Thursday night and, as often happens at this latitude we were visited next day by King Neptune and his beautiful lady wife together with a retinue of judge, barber, doctor, and several policemen.
We were advised that various members of our party had been identified as ‘pollywogs’ - innocents who had not crossed the line previously and needed to be tried for crimes against Neptune.
Following time-honoured custom we then ran off to hide but were found one by one by the police and taken to the court for trial – where all the miscreants were found guilty, ceremonially shaved, given a few slops, injected with rather vile medicine, and invited to ‘kiss the kipper’.
Jeremy having made little effort to hide was caught rather early on, judged to have been particularly disrespectful and punished for numerous misdemeanours, which was more or less fair. But, later he was given a second trial for heckling, which was a bit mean.
Martine by contrast hid with supreme ingenuity and nearly escaped the process completely. However, she was eventually found and dragged to the court. Here she showed great aplomb but was found guilty of an exceptionally long and serious list of crimes (including being French and claiming to have crossed the line on a foreign ship) and got a well-deserved punishment.
The police then decided that one of their number, the electrician Johnie needed a little special treatment, as he had only previously crossed the line on cruise ships, which doesn’t count. After that the pollywogs managed to turn the tables, staging a revolt and successfully chastising the doctor and various police.
Finally we rounded off with a bit of a party, which felt well-earned as we have been working more or less non-stop for the past three weeks, and to allow this the next morning’s early station was cancelled.
Apparently this is something of a tradition on AMT cruises and means that there is a bit of a gap in our data set just around the equator.
Jeremy and Martine, now in the South Atlantic
Jeremy, Tuesday, October 28th, 2008
5.15 Alarm goes off, Martine and I have worked out we don’t need to both get up early so we are alternating this, and unfortunately it is my turn for an early morning.
After getting up I nip across to the bar, conveniently just around the corner from my cabin, for a coffee. Then down to the lab where one of the Plymouth Marine Lab scientists, Carolyn, and chief scientist Malcolm, show me a large black insect that was found on the deck this morning. This is rather strange as we are more than a thousand miles from land.
Since I come from the Natural History Museum they hope I may be able to help identify it. It looks like a big black grasshopper to me; I take a set of photos and promise to ask an expert.
5.45 As I finally get to our corner of the lab the CTD and rosette sampler are still going down on its water collecting mission so I have time to wash and rinse our bottles and prepare the syringe filters we are using to take reconnaissance samples.
6.15 The CTD comes back on deck so it is time to collect the water – attaching hoses to the bottles on the sampler to fill up our jerry cans. This is really the only outdoor job we have so one of our favourite tasks. The sun comes up as we are sampling and a big frigate bird comes to visit, circling over the ship a few times.
6.45 Sampling over, it is back into the lab to do the syringe filter preps and start the main filtration process going.
7.30 Breakfast – a very welcome cooked breakfast (and more coffee), we are well looked after on the ship.
8.00 Start the main work of the morning: Processing the filter samples generated from the day before - we are collecting from 9 water depths in the morning and 13 in the afternoon.
There are two filters from each depth, as we are taking different filter types for light and electron microscopy, so there is a constant flow of filters to look after.
The filters for electron microscopy simply need to be transferred into petri-slides and labelled. For the light microscopy filters, I also make up microscope slides.
9.00 Take a break to send an email to a couple of entomologists in the museum to ask what the grasshopper is. I can’t remember who specialises in this group but I am very confident someone will know (especially as it is a big distinctive insect).
Then back to the microscope to check on the samples I collected this morning. We are near the equator now, the sea is getting more productive, and the coccolithophore communities are slowly changing – especially, and this is something of a surprise, the communities at intermediate depths around 50m.
10.00 Get an email reply from George Beccaloni explaining that our grasshopper is ‘an adult female Mediterranean Field Cricket, Gryllus bimaculatus. This species has a very wide distribution from Africa to Asia and it is also bred extensively in captivity for live food for reptiles etc, and as a lab animal. This species may have flown on board, but it may also be a stowaway. I had one recently brought in to the museum which jumped out of someone’s suitcase in the UK when they got back from Egypt.’
The fact that it is a common species is something of a relief, since Carolyn was very unhappy with the idea of me preserving him for the museum collections – a dragonfly which died on the deck a couple of days ago has ended up pickled in the fridge.
10.45 Reconnaissance of these morning’s filters is finished so I complete the sample labelling and take the trays down to the lab to start filling up with the next set of filters.
Start a more detailed examination of the afternoon filters from yesterday – the basic aim is to do a count of coccolithophore abundance in all the samples and note the dominant species but for the afternoon samples I am usually able to do a more detailed analysis.
12.00 Lunchtime – like I said they look after us well and lunch is a three course meal - with quite a bit of discussion of the grasshopper and other visitors. Then a half hour break for a relaxed coffee in the bar and a little wander round the ship.
12.45 Prepare for the afternoon sampling. Martine has decided that this is a good time for an extended sampling so we can collect bulk organic matter samples for DNA analysis through the water column. This means pressing all our bottles back into service.
After washing the bottles, time for a bit of a stroll and chat as the CTD goes out. The lunchtime (or more technically ‘solar noon’) halt is rather impressive as in addition to the CTD midships there is a plankton net deployed further forward and an optics rig near the stern so with three cranes out, the ship looks like it is undertaking large-scale fishing.
13.30 The CTD comes out of the water and sampling gets back in earnest. With about 120 litres of water to collect there is a lot to do – and it is hot and humid outside.
14.00 After half an hour of work in the sun the samples are safely lined up in the lab, and I am in severe need of a shower.
14.30 Back to the microscope to finish off the analysis of yesterday afternoon’s samples – there is a lot of really nice stuff in the samples, but the day is getting a bit long and around this time I find microscope work pretty hard going; a bit of music helps and luckily there is an impressive system in the UIC (Underway Instrumentation Centre) where we have set-up the microscopes.
16.00 Start my turn of the day’s filter work. Filtering water is pretty much the coalface of oceanography and there is a lot of it going on on the ship. The basic principle is that you pour water into the top of the filtration apparatus it gets pumped through the filter and into a receiving carboy and the sample gets left behind on the filter.
So the filtering process basically involves pouring water in, ticking off how much has been filtered, watching till the water level falls near the bottom (running the filter dry is a bad idea), closing the tap, pouring in more, re-opening the tap and so on in (hopefully) a nice steady rhythm for as many hours as it takes to get all the samples done.
17.00 Science meeting – there are 18 scientists on board, and although we are in separate small teams, it is very useful to meet once a week or so to discuss plans for the next few days sampling, and debate any interesting results (are low surface-water salinities here a result of Amazon outflow?). Today’s meeting also includes a briefing on the crossing the line ceremony that is getting close and becoming a major focus of discussion.
17.20 Back to the filtration – rather tranquil as the sun is low on the horizon and comes in through the lab windows, through which every now and then I can just about see a shoal of flying fish jumping out of the water.
Further away, there is less tranquility, as the engineering officers have got out their new inflatable swimming pool.
This is tempting but Martine and I still have a lot of work to do as a result of the extended sampling today.
18.20 Stop the filtration, change for dinner (no t-shirts or shorts allowed), and, as the sun is now definitely over the yardarm, its time for a medicinal G&T in the bar.
19.30 Time for an after dinner coffee and chat in the bar – the bar has a bar, obviously, but it also has lot of comfortable seating space, tables, a darts board etc. and is the social centre for the scientists and officers (there is a separate crew bar which seems a touch anachronisitic but no-one on the ship wants to change that).
After dinner is probably the busiest time of day in the bar with most of us there, a lot of open discussion, and usually a group attempt at doing the crossword from the shipboard paper. Later on the numbers decrease as many people are working shift systems, or retire to their cabins, or like Martine and I tonight, have work to finish off.
20.15 Finish the last bit of filtering then spend an hour or so picking through the day’s zooplankton sample for pteropods– this is a particularly pleasant and relaxing task which I will have to explain in another blog entry.
21.30 Rejoin the bar group for a wind down at the end of a rather longer than average day.
Jeremy, nearly at the equator.
Martine, Tuesday, October 21st, 2008
Sunday 19th October, 10 pm
I’m outside by a nice clear night, writing from the hammock set up out of my cabin. It is the end of the weekend and there are not many people awake onboard… apart from Alex (the third mate) and Kevin (watchman) at the bridge to check there is nothing in our way and everything is working fine.
It’s Sunday evening, however there is no real weekend onboard as we are sampling everyday. This weekend was especially busy with a deep sampling, down to 5150m on top of the normal 2 stations. We even ran out of containers to collect the extra samples and had to use the collapsed carboys.
This supplementary collection means for Jeremy and I about 290 litres of seawater filtered instead of the usual 160 litres per day, and about 20 extra slides to look at during the day… But it also means souvenirs as we sent down a team of expanded polystyrene cups attached at the top of the rosette sampler in socks (special thanks to Glen and Jeremy who provided the socks).
At 5000m the pressure is about 600 times that at the surface and our valiant team was shrunk to a fraction of their previous size – this also meant that our swift marker-pen cartoons were rendered into finely detailed pieces of art.
Busy and fertile because most of the samples we collected over the weekend were really nice, and quite distinctive and thus worth having! We also had a nice barbecue-style party on the deck in the evening to relax and enjoy.
After dinner in the very dark night (no moon) some of us had the chance to observe bioluminescence (pictures to come later if we remember a camera next time) around the ship, the best observation spot was at the bow on the forecastle. The galley telegraph subsequently carried rumours of nocturnal entertainments near the kitchen (I’ve no idea what that was about, honest)…
Martine 11.13°N 32.06°W
Jeremy, Wednesday, October 15th, 2008
I am writing this from Monkey Island – the open space on top of the bridge where we can watch the world go by. And right now I am surrounded on all sides by absolutely nothing. There are no islands, no ships, no dolphins, no birds, just gently rolling dark blue water in all directions, except up. The complete absence of everything is because we have now got near to the centre of the North Atlantic gyre, which is one of the biggest marine deserts on earth.
Of course, unlike the Sahara desert, which is 2000 miles due east of us, there is no shortage of water here. But that water contains very very little of certain key elements that life needs to grow, especially nitrogen and phosphorus (the same nutrient elements as I should be adding to my lawn back home to get the grass to grow a bit better next summer). Any trace of these elements in the water gets hoovered up by plant plankton.
Now, you would expect that the nice marine ecosystem would sensibly recycle these elements. However, the ecosystem is in fact hopelessly inefficient and when they die the plankton sink out of the water column taking the nutrients with them. So, the sunny wind-mixed top 60m or so of the ocean is almost lifeless.
Almost but not quite - some specialist plant-plankton have adapted to this harsh environment including particularly many of our friends the coccolithophores, the cacti of the oceanic deserts.
So, Martine’s filtering is yielding a wonderful array of exotic specimens for the microscope. From the surface layers we need to pump several litres of sea-water to get enough to find on our filters. Deeper down, where the water is more nutrient-rich, different specialist forms adapted to low light levels occur at slightly higher abundances, although down there they have a range of things to compete with including cheerily-named cyanobacteria which several other people are studying on the cruise (more on them, the people not the cyanobacteria, another time).
As we sail south each station has a slightly different water column structure and ecology. Observing the changing coccolithophore communities in the field is both a great way to ensure we get the possible results and extremely rewarding – coccolith heaven, I am back to the microscope.
Martine, Monday, October 13th, 2008
Already a week since we left the stable land for the not-so-quiet sea. Time flies out here even though we have already gained 2 extra hours by sailing west, towards the sunset.
After a rough beginning of the week, the weather cleared up and the sea is more gentle now. We have settled into a sampling routine, usually with 2 sampling stations a day, one before dawn (unnaturally early according to Jeremy), the other at midday.
Sampling begins with the rosette sampler being lowered down through the upper 300m of water. It carries 24 bottles, each of which has an individual firing system to collect the seawater at specific depths.
Because the weather is now better, we can secure the rosette on the outside deck between samplings. This makes our life much easier when it comes to recovering the water. When the weather was rough, it had to be secured as soon as possible in its ‘cabin’ where it fits tightly, and half a dozen scientists would try to get in as well to recover their samples.
After recovering the seawater, our main task is to filter it. We have to do this in several different ways depending on what we are trying to find out. First of all we look at a small syringe filter under the microscope to decide the volume of water to filter. We use membranes with holes that are 0.2 or 1 microns wide (a 10,000th of a centimete) to filter the water. The process takes place in a vacuum and we use a recovering flask called a carboy.
While Jeremy set up the microscope in the dry lab, I played with tubing in the wet lab to set up our filtering system. Unfortunately our carboys were not delivered in time, so I tried (unsuccessfully) to use a fermenting cask. It collapsed. Then I tried a thick plastic flask. It also collapsed. We managed to borrow a 20 litre heavy duty carboy from Glen Tarran (a colleague from Plymouth). Fingers crossed, this one is still alive and healthy…
Because our 2 filtering ramps are on 2 different benches I had fun playing with tubing, connections and taps to connect everything. The best thing is that Jeremy loves to come and watch all the bubbly tubing!
We have now tried all our protocols and are ready for intensive sampling to produce LM (Light Microscopy) and SEM (Scanning Electron Microscope) slides, bulk and probe DNA samples, and other samples, during the second part of the cruise, south of the Azores.
Martine, off the Azores
Jeremy, Monday, October 6th, 2008
We’re off. After several weeks of increasingly hectic shopping, and other preparations, we are now sailing down the English Channel (well actually we have stopped off at the Isle of Wight to practice sampling).
The photo below shows our equipment and supplies being winched onto the James Clark Ross. The pile looked very large as it was filling first Martine’s office in the Museum then the car (in fact it only just fitted) but it looked minute on the quay next to the ship, and insignificant next to everything else being loaded onboard.
The main reason the James Clark Ross goes south each year is to resupply the British Antarctic Survey bases and the ship is carrying tonnes of equipment and supplies for the scientists in the Antarctic. Mostly it is in crates but there is some pretty cool stuff on deck. In addition there is the party of eighteen scientists, including ourselves, for the slow cruise down to the Falkland Islands studying ocean life and processes as we go and they have a lot more equipment.
Once our little pile of gear had been put on the ship, we then had a day and a bit to unpack, set-up, and fix everything down securely – lots of drilling, screwing, and strapping. We also of course had to nip out and buy a whole lot more stuff, but we are now safely isolated from all shops so Martine will have to make do with whatever we have got.
Actually the shopping trip was curtailed by the need to get back for the safety lecture where we learnt what to do when the ship sinks (unlike the Titanic there are as many lifeboat places as people on board). Then on Friday, after one more lifeboat drill, we sailed out of Immingham docks (just next to Grimsby) and down through a surprisingly lively North Sea.
Jeremy, The English Channel