NIU Anou and FENG Youren
Department of Parasitology,
Tongji Medical College, Huazhong University of Science and Technology
(Wuhan, China 430030)
The usefullness of random amplified polymorphic DNA markers (RAPD) was assayed in order to discriminate among species, strains and sexes within the genus schistosoma. Six arbitary decamer oligonucleotides were used as primers to amplify total DNA by the polymerase chain reaction (PCR). Hightly variable product patterns were observed between S.mansoni and S.japonicum by all primers. Some of the tested primers, for example B5, A7, produced patterns included bands that were polymorphism between strains of mainland and Taiwan in China, other primers, for example B6, produced apparently identical produced patterns. Minor differences were observed between male and female adult, fragment of 718 bp was reported as six markers. Data were analyzed by Nei's genetic distance (D), and genetic similarity (S). Nei's genetic distance (D) between two species was more than 0.90; Nei's genetic distance (D) between mainland and Taiwan strains of S.japonicum was more than 0.20; 6 strains from marshland-lake regions, they were ranged from 0.000 to 0.029, between marshland lake regions and mountainous-hilly regions ranged from 0.060 to 0.067. A phylogenetictree was outlined using SAS version 6.12 computer program. The results already indicated clearly that RAPD markers constitute a powerful tool for the analysis of genetic variability. Mainland and Taiwan in China S.japonicum strains have taken place intra-specific variation. The results indicated that the random amplification of polymorphism DNA (RAPD) may be an extremely useful approach to identification of Schistosoma strains, species and sexes, and the results also indicated that Schistosoma in China had occurred genetic diversity among different geographical strains.
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created 21/12/00
