XIAO-GUANG Chen1 , MING-CHIU Fung2 , XIAO-HONG Zhou1 , HUA Li1 and SHU-MAN Shen1
1 Department of Parasitology,
Institute of Tropical Medicine, The First Military Medical University,
Guangzhou 510515, China
2 Department of Biology,
The Chinese University of Hong Kong
The cDNA library constructed from Schistosoma japonicum cercariae was firstly reported. Total RNA extracted from 500,000 S.japonicum cercaria was used to syntheses the double-stranded cDNA and cloned into the lTriplEx2 vector using "SMART cDNA Construction Kit". The primary titer of the constructed cDNA library is 1.8 x 107 pfu and the titer of amplified library is 2.5 x 1010 pfu/ml. The average size of inserts is 1.075 kb. The recombinant efficiency is 94.4%. The full length cDNA of S. japonicum TPI and JF-2 genes were successfully amplified from the library. All the data showed that a representative cDNA library of S. japonicum cercariae has been constructed. The cDNA library constructed from S. japonicum cercariae was firstly reported. Total RNA extracted from 500,000 S.japonicum cercaria was used to syntheses the double-stranded cDNA and cloned into the lTriplEx2 vector using "SMART cDNA Construction Kit". The primary titer of the constructed cDNA library is 1.8 x 107 pfu and the titer of amplified library is 2.5 x 1010 pfu/ml. The average size of inserts is 1.075 kb. The recombinant efficiency is 94.4%. The full length cDNA of S. japonicum TPI and JF-2 genes were successfully amplified from the library. All the data showed that a representative cDNA library of S. japonicum cercariae has been constructed.
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created 21/12/00
