Preliminarily Screening Potential Protective Antigen from Schistosoma japonicum Cercariae

LIU Guozhang¹, XIE Wenyue² and CHEN Xiaoguang^1
1Dept. Parasitology, the First Military Medical University, Guangzhou 510515 China.
²Dept. Clinical Lab., General Military Hospital, Guangzhou 510010 China.

Sera from rabbits vaccinated mutiplly with ultraviolet-attenuated cercariae of Schistosoma japonicum were used to screen novel effective protective antigen against Schistosoma. Comparing soluble cercariae antigen(SCA) with soluble adult worm antigen(SAWA), Western blot analysis suggested that the epitopes recognized on the Mr>97Kda antigen both in SCA and SAWA.The epitopes recognized on Mr 215 Kda,195 Kda,170 Kda,155 Kda,140 Kda and so on were shown to be cross-reacted with both immunized sera and infected sera whereas immunized sera specifically recognized a SCA 104 KDa and a SCA 60 KDa antigen which showed an enhanced reactivity with immunized rabbit sera in comparison with infected rabbit sera. No SAWA antigen were recognized specially. Thus, SCA104KDa and SCA60KDa were screened preliminarily and might be correlated with protection against S.japonicum. Further research is needed for the two potential protective antigen candidates.

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created 21/12/00