CUI Yu, CHENG Xiaoxin, WANG Ying
and ZHEN Lili
Department of Parasitology,
Dalian Medical University, Dalian 116023. e-mail : jsc@mail.dlmedu.edu.cn
AIM: The paper observed
the different levels and content of XOD, MDA and GSH in the different
organ tissues and sera from mice infected by T. spiralis
in order to probe the oxidative damage of multi-organ tissues
and formation of trichinosis. We finally understood the pathogenesis
of trichinosis.
METHOD: The experiment was randomly divided into four examined groups and a controled group. There were six mice in each group. The examined mice fed to capsules with living larva of T. spiralis, and that controled mice fed to normal mice meat before experiment. We collected the intestinal tissues, hepatic tissues, cardiac tissues, brain tissues, lung tissues and kidney tissues as well as blood to centrifugate on 3, 7, 14, 21, 30 day post infection, and then keeping examined materials into the refrigerator. The different levels and content of XOD, MDA and GSH in the above examined materials and sera were determined by those methods introduced in the reference.
RESULT and DISCUSSION: These results showed that the level
and content of MDA in intestinal tissues and the levels and content
of MDA and XOD in hepatic tissues in infected mice were higher
significantly than that in controled groups from 3_7 days after
infection (p<0.01), the levels of GSH were significantly lower
than that in control groups at the same time (p<0.01). these
results indicated the oxidative damage of intestinal tissues and
hepatic tissues had produced in early stage of trichinosis. After
14 days, the level of GSH in intestinal tissues gradually got
back normal level, the level of MDA firstly fell off and then
went up again. The level of XOD in hepatic tissues also returned
gradually, but the levels of MDA and GSH went up constantly and
kept higher level. The level of XOD in cardiac tissues obviously
rose after 14 days, and reached to peak after 30 days. MDA firstly
rose and then fell down. GSH rose significantly (p<0.05) after
30 days, at the same time MDA in brain tissues and sera kept higher
level. These results in this stage suggested the oxidative damage
was transfered to visceral organic tissues from intestinal and
hepatic tissues with change of live cycle of T. spiralis,
and showed the oxidative damage started to focus to important
visceral organs. The levels of GSH and MDA of brain tissues showed
reverse change from 14 to 30 days. The level of MDA in sera was
steadily maintained at a higher level.
CONCLUSION: These results indicated that T. spiralis could induce free radicals to damage some organs (and tissues of mice with trichinosis), and oxidative metabolites were so accumulated that toxemia was formed. The two pathogenic factors might be closely related to the happening and development of trichinosis.
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created 21/12/00
