JIANG Mingsen, DONG Huifen, MING
Zhenping and ZHONG Qinping
Department of Medical Parasitology
and Research Laboratory of Schistosomiasis, Medical College of
Wuhan University, Wuhan 430071
AIM: To study the effects of EGF on the growth and proliferation of cultured cells from adult Schistosoma japonicum.
METHOD: After the cells collected from the adult Schistosoma japonicum were cultured in medium RPMI-1640 containing 20% calf serum and a moderate amount of antibiotics for 3 days, they were divided into two groups. The cells in the first group were treated directly by EGF with concentrations of 0, 0.5, 1, 2, 4, 8, 12, 16, 20, 24 and 28ng/ml, respectively. The cells in the second group were designed to treat by MNNG of 3mg/ml for 48 hours before they cultured in the medium with EGF of 0, 1, 8, 12, 16 and 20ng/ml, respectively. The growth and proliferation of cultured cells were observed by microscope everyday.
RESULTS: Some of the cultured cells in the medium with different concentration of EGF fell off and degenerated in both groups after two weeks. Moreover, along with the increase of the EGF concentration in the medium, the abscission and degeneration of the cells became serious. Cultured cells in the first group fell off or degenerated earlier than those in the second group.
CONCLUSION: EGF cannot induce the proliferation of the cultured cells from adult Schistosoma japonium. On the contrary, it can accelerate the degeneration of the cultured cells, especially for the cells not treated by MNNG.
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created 21/12/00
