Studies on the dipstick of schistosomiasis japonica

JIANG Changfu, SHI Youen and GAN Yin
Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030

In this study , "Studies on the fast diagnose test paper of Schistosomiasis japonica", the Schistosoma japonicum soluble egg antigen (SEA), the S.japonicum adult worm membrane antigen (WMAg), cercaria antigen (CeAg) , recombinant antigens and the anti-SEA, anti-SEA140kDa, anti-SWAg, anti-GAA, anti-CCA, anti-id-McAb were extracted, purified and screened by the techniques of the Modern Molecular Biology, the immunology and the genic engineering. These purified proteins were used in the studies of the Valkiris' dot-immunogold filtration assay (DIGFA) and the dot-immunochromatographic assay (DICA) after immunogold mark. The results showed that this is a useful method for the quickly serological diagnosis of schistsomiasis japonica, which is named as Colloidal Gold Sj-DIPSTICK. This method was employed for detecting 325 cases with shistosomiasis (28 cases with acute shistosomiasis and 297 cases with chronic schistosomiasis were verified by the miracidium hatching test), 153 cases with paragonimiasis, 79 cases with clonorchiasis, 157 cases with cysticercosis, 184 cases with trichinelliasis, 165 cases with hepatitis, 481 cases with pulmonary tuberculosis, 876 cases from the special clinic of our department and 518 healthy persons. And it was compared with some regular detective methods. Both suggested that the Colloidal Gold Sj-DIPSTICK is more qualified.

The reasons are:

1. Simple: The method needs no apparatus, is suitable in the primary hospital and the field surveys
2. Quick: The reaction can be performed in 2-5 minutes
3. High sensitivity: The positive detective rate is as high as 97.31-100%
4. High specificity: The cross-reactions were less than 5%(0.62-4.89%). The rates were 1.96% with paragonimiasis, 1.33% with clonorchiasis, 0.64% with cysticercosis, 4.89% with trichinelliasis, 1.21% with hepatitis and 0,62% with pulmonary tuberculosis , respectively
5. Low false positive rates: The false positive rates were 0.19% with the healthy persons and 1.25% with the cases from the special clinic of our department.

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created 21/12/00