DONG Huifen, JIANG Mingsen, MING
Zhenping and ZHONG Qinping
Department of Medical Parasitology
and Research Laboratory of Schistosomiasis, Medical College of
Wuhan University, Wuhan 430071
AIM: To study the function of MNNG on the proliferation of cultured cells from adult Schistosoma japonicum.
METHOD: After the cells collected from the adult schistosome were cultured in medium RPMI-1640 containing 20% calf serum and a moderate amount of antibiotics for five days, they were divided into two groups. The cells in the first group were treated by MNNG with concentrations of 0, 1, 2, 3, 4, 6 and 9mg/ml for 0, 24, 36 and 48 hours, respectively. The cells in the second group were designed to control group. Afterwards all cells were moved into the original medium. Three weeks later, the rate of calf serum in the medium was adjusted into to 5%. The proliferation and growth of the treated cells were observed by microscope everyday.
RESULT: Most of the cells treated by MNNG with concentrations of 6 and 9mg/ml fell off from the wall of cultured flasks after 3 days, and the remained cells cultured in the medium containing 5% calf serum declined gradually. All the cells treated by MNNG of 1, 2, 3 and 4mg/ml grew well in the medium. The volume of the cells was larger and more cells in division were observed in the first group than in the control. Especially in the cells treated by MNNG of the concentration of 3mg/ml for 48 hours.
CONCLUSION: MNNG with the moderate concentration can induce the proliferation and division of cultured cells from adult Schistosoma japonicum.
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created 21/12/00
